Any feedback?
Literature summary for 5.4.99.16 extracted from
- Gene cloning, expression, and characterization of a novel trehalose synthase from Arthrobacter aurescens (2009), Appl. Microbiol. Biotechnol., 83, 477-482.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Paenarthrobacter aurescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 98% residual activity at 5 mM | Paenarthrobacter aurescens |  |
| Cu2+ | complete inhibition at 5 mM | Paenarthrobacter aurescens | |
| EDTA | 86% residual activity at 5 mM | Paenarthrobacter aurescens | |
| Mg2+ | 92% residual activity at 5 mM | Paenarthrobacter aurescens | |
| Mn2+ | 86% residual activity at 5 mM | Paenarthrobacter aurescens | |
| SDS | complete inhibition at 1 mM | Paenarthrobacter aurescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 123% relative activity at 1 mM | Paenarthrobacter aurescens | |
| Mg2+ | 118% relative activity at 1 mM | Paenarthrobacter aurescens | |
| Mn2+ | 116% relative activity at 1 mM | Paenarthrobacter aurescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
SDS-PAGE | Paenarthrobacter aurescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter aurescens | B8YM30 | - |
- |
| Paenarthrobacter aurescens CGMCC 1.1892 | B8YM30 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Paenarthrobacter aurescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
Paenarthrobacter aurescens | alpha,alpha-trehalose | as a byproduct, about 13% glucose is also produced | r | |
| maltose | - |
Paenarthrobacter aurescens CGMCC 1.1892 | alpha,alpha-trehalose | as a byproduct, about 13% glucose is also produced | r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
Paenarthrobacter aurescens |
| TreS | - |
Paenarthrobacter aurescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Paenarthrobacter aurescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | the enzyme remains stable up to 40°C, and shows complete loss of activity at 60°C | Paenarthrobacter aurescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Paenarthrobacter aurescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
Paenarthrobacter aurescens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Paenarthrobacter aurescens | expression induction with 0.4 mM IPTG at 25°C for 5 h is the best condition for the expression of soluble TreS, higher temperature or IPTG concentrations lead to a higher rate of inclusion bodies, whereas a temperature below 20°C and lower IPTG concentrations decreases the total amount of TreS | up |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
