Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycolicibacterium smegmatis |
expressed in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the MTase activity of TreS is inhibited by 5 mM Ca2+ and other divalent cations | Mycobacterium tuberculosis | |
Ca2+ | the MTase activity of TreS is inhibited by 5 mM Ca2+ and other divalent cations | Mycolicibacterium smegmatis | |
additional information | not inhibited by acarbose | Mycobacterium tuberculosis | |
additional information | not inhibited by acarbose | Mycolicibacterium smegmatis | |
validoxylamine | strong inhibition | Mycobacterium tuberculosis | |
validoxylamine | strong inhibition | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
maltose | in 40 mM potassium phosphate buffer (pH 6.8), at 37°C | Mycolicibacterium smegmatis | |
90 | - |
alpha,alpha-trehalose | in 40 mM potassium phosphate buffer (pH 6.8), at 37°C | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
6 * 65000, SDS-PAGE | Mycolicibacterium smegmatis |
65000 | - |
6 * 65000, SDS-PAGE | Mycobacterium tuberculosis |
68000 | - |
6 * 68000, gel filtration | Mycolicibacterium smegmatis |
68000 | - |
6 * 68000, gel filtration | Mycobacterium tuberculosis |
390000 | - |
gel filtration | Mycolicibacterium smegmatis |
390000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni column chromatography | Mycobacterium tuberculosis |
Ni column chromatography, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, aminohexylagarose column chromatography, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | - |
Mycolicibacterium smegmatis | alpha,alpha-trehalose | - |
r | |
maltose | - |
Mycobacterium tuberculosis | alpha,alpha-trehalose | - |
r | |
additional information | TreS has also amylase activity by producing trehalose from glycogen or maltoheptaose | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | TreS has also amylase activity by producing trehalose from glycogen or maltoheptaose | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 65000, SDS-PAGE | Mycolicibacterium smegmatis |
homohexamer | 6 * 65000, SDS-PAGE | Mycobacterium tuberculosis |
homohexamer | 6 * 68000, gel filtration | Mycolicibacterium smegmatis |
homohexamer | 6 * 68000, gel filtration | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
MTase | - |
Mycolicibacterium smegmatis |
MTase | - |
Mycobacterium tuberculosis |
Trehalose synthase | - |
Mycolicibacterium smegmatis |
Trehalose synthase | - |
Mycobacterium tuberculosis |
TreS | - |
Mycolicibacterium smegmatis |
TreS | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
validoxylamine | in 40 mM potassium phosphate buffer (pH 6.8), at 37°C | Mycolicibacterium smegmatis |