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Literature summary for 5.4.99.16 extracted from
- Gene cloning, expression, and biochemical characterization of a recombinant trehalose synthase from Picrophilus torridus in Escherichia coli (2006), J. Agric. Food Chem., 54, 7098-7104.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Picrophilus torridus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 1 mM, complete inhibition | Picrophilus torridus |  |
| Hg2+ | 1 mM, complete inhibition | Picrophilus torridus | |
| sodium dodecylsulfate | 1 mM, complete inhibition | Picrophilus torridus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 42.4 | - |
maltose | 45°C, pH 6.0 | Picrophilus torridus | |
| 137.8 | - |
maltose | 45°C, pH 6.0, presence of 10 mM glucose | Picrophilus torridus | |
| 210.3 | - |
trehalose | 45°C, pH 6.0 | Picrophilus torridus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Picrophilus torridus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Picrophilus torridus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Picrophilus torridus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80 | - |
45°C, pH 6.0 | Picrophilus torridus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose | ratio of kcat to Km value is 2.5fold higher for maltose than for trehalose | Picrophilus torridus | maltose | - |
r | |
| maltose | ratio of kcat to Km value is 2.5fold higher for maltose than for trehalose. Maximum conversion rate for maltose into trehalose is independent of substrate concentration and reaches 71% at 20°C | Picrophilus torridus | alpha,alpha-trehalose | - |
r | |
| sucrose | low activity on sucrose | Picrophilus torridus | D-glucose + D-fructose + [alpha-D-glucopyranosyl-(1,1)-D-fructofuranose] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Picrophilus torridus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Picrophilus torridus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pH 6.0, stable for 20 min | Picrophilus torridus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 95 | - |
maltose | 45°C, pH 6.0 | Picrophilus torridus | |
| 97.8 | - |
maltose | 45°C, pH 6.0, presence of 10 mM glucose | Picrophilus torridus | |
| 187.6 | - |
trehalose | 45°C, pH 6.0 | Picrophilus torridus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Picrophilus torridus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | more than 80% of maximum activity within this range | Picrophilus torridus |
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