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Literature summary for 5.4.99.15 extracted from
- Bifunctional recombinant fusion enzyme between maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase of thermophilic microorganism Metallosphaera hakonensis (2008), J. Microbiol. Biotechnol., 18, 1544-1549.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)pLysS cells as fusion enzyme with maltooligosyltrehalose trehalohydrolase | Metallosphaera hakonensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
MTSH fusion enzyme | Metallosphaera hakonensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metallosphaera hakonensis | - |
strain JCM8857 | - |
| Metallosphaera hakonensis JCM 8857 | - |
strain JCM8857 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltohexaose | - |
Metallosphaera hakonensis | alpha-maltotetraosyl trehalose | - |
? |  |
| maltohexaose | - |
Metallosphaera hakonensis JCM 8857 | alpha-maltotetraosyl trehalose | - |
? | |
| maltohexaose | - |
Metallosphaera hakonensis JMC8857 | alpha-maltotetraosyl trehalose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| maltooligosyltrehalose synthase | - |
Metallosphaera hakonensis |
| MTS | - |
Metallosphaera hakonensis |
| MTSH | fusion enzyme consisting of maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase | Metallosphaera hakonensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
MTSH fusion enzyme | Metallosphaera hakonensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | the MTSH fusion enzyme exhibits a high degree of thermostability, retaining 80% of the activity when pre-incubated at 70°C for 48 h, the stability is gradually abolished by incubating the fusion enzyme at above 80°C for 30 min | Metallosphaera hakonensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | MTSH fusion enzyme | Metallosphaera hakonensis |
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Release 2023.1 (January 2023)
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