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Literature summary for 5.4.99.15 extracted from

  • Kato, M.; Miura, Y.; Kettoku, M.; Shindo, K.; Iwamatsu, A.; Kobayashi, M.
    Purification and characterization of new trehalose-producing enzymes isolated from the hyperthermophilic archae, Sulfolobus sulfataricus KM1 (1996), Biosci. Biotechnol. Biochem., 60, 546-550.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
x * 76000, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharolobus solfataricus enzyme is involved in the pathway of trehalose synthesis from starch ?
-
?
additional information Saccharolobus solfataricus KM1 enzyme is involved in the pathway of trehalose synthesis from starch ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
KM1
-
Saccharolobus solfataricus KM1
-
KM1
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
192
-
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Maltopentaose
-
Saccharolobus solfataricus alpha-Maltotriosyl trehalose
-
?
Maltopentaose
-
Saccharolobus solfataricus KM1 alpha-Maltotriosyl trehalose
-
?
additional information enzyme is involved in the pathway of trehalose synthesis from starch Saccharolobus solfataricus ?
-
?
additional information enzyme is involved in the pathway of trehalose synthesis from starch Saccharolobus solfataricus KM1 ?
-
?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 80
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
6 h, 9% loss of activity Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
Saccharolobus solfataricus