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Literature summary for 5.4.99.13 extracted from

  • Cracan, V.; Padovani, D.; Banerjee, R.
    IcmF is a fusion between the radical B12 enzyme isobutyryl-CoA mutase and its G-protein chaperone (2010), J. Biol. Chem., 285, 655-666.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paraburkholderia xenovorans
expressed in Escherichia coli BL21(DE3) cells Geobacillus kaustophilus
expressed in Escherichia coli BL21(DE3) cells Nocardia farcinica

Inhibitors

Inhibitors Comment Organism Structure
GDP the presence of GDP decreases kcat about 1.6-1.7fold while increasing the Km about 2fold. Consequently, the catalytic efficiency kcat/Km value of IcmF decreases 3fold in the presence of GDP Geobacillus kaustophilus
GTP the presence of GTP decreases kcat about 1.6-1.7fold while increasing the Km about 2fold. Consequently, the catalytic efficiency kcat/Km value of IcmF decreases 4.5fold in the presence of GTP Geobacillus kaustophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0201
-
 isobutyryl-CoA recombinant enzyme, at 37°C Geobacillus kaustophilus
0.0453
-
 isobutyryl-CoA recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C Geobacillus kaustophilus
0.0508
-
 isobutyryl-CoA recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C Geobacillus kaustophilus

Organism

Organism UniProt Comment Textmining
Geobacillus kaustophilus
-
-
-
Nocardia farcinica
-
-
-
Paraburkholderia xenovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration Paraburkholderia xenovorans
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration Geobacillus kaustophilus
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration Nocardia farcinica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.34
-
 recombinant enzyme, at 37°C Paraburkholderia xenovorans
0.75
-
 recombinant enzyme, at 37°C Geobacillus kaustophilus
1.1
-
 recombinant enzyme, at 37°C Nocardia farcinica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isobutyryl-CoA reversible interconversion Paraburkholderia xenovorans n-butyryl-CoA
-
 r
isobutyryl-CoA reversible interconversion Geobacillus kaustophilus n-butyryl-CoA
-
 r
isobutyryl-CoA reversible interconversion Nocardia farcinica n-butyryl-CoA
-
 r

Synonyms

Synonyms Comment Organism
ICM
-
 Paraburkholderia xenovorans
ICM
-
 Geobacillus kaustophilus
ICM
-
 Nocardia farcinica
IcmF a fusion between isobutyryl-CoA mutase and its G-protein chaperone Paraburkholderia xenovorans
IcmF a fusion between isobutyryl-CoA mutase and its G-protein chaperone Geobacillus kaustophilus
IcmF a fusion between isobutyryl-CoA mutase and its G-protein chaperone Nocardia farcinica
isobutyryl-CoA mutase fused a fusion between isobutyryl-CoA mutase and its G-protein chaperone Paraburkholderia xenovorans
isobutyryl-CoA mutase fused a fusion between isobutyryl-CoA mutase and its G-protein chaperone Geobacillus kaustophilus
isobutyryl-CoA mutase fused a fusion between isobutyryl-CoA mutase and its G-protein chaperone Nocardia farcinica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme is unstable at 60°C Geobacillus kaustophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.88
-
 isobutyryl-CoA recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C Geobacillus kaustophilus
1.96
-
 isobutyryl-CoA recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C Geobacillus kaustophilus
3.1
-
 isobutyryl-CoA recombinant enzyme, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C Geobacillus kaustophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
37
-
 isobutyryl-CoA recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C Geobacillus kaustophilus
43
-
 isobutyryl-CoA recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C Geobacillus kaustophilus
150
-
 isobutyryl-CoA recombinant enzyme, at 37°C Geobacillus kaustophilus