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Literature summary for 5.4.99.13 extracted from

  • Ratnatilleke, A.; Vrijbloed, J.W.; Robinson, J.A.
    Cloning and sequencing of the coenzyme B(12)-binding domain of isobutyryl-CoA mutase from Streptomyces cinnamonensis, reconstitution of mutase activity, and characterization of the recombinant enzyme produced in Escherichia coli (1999), J. Biol. Chem., 274, 31679-31685.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
IcmB, expression in Escherichia coli, IcmB shows significant identity to several cobalamin-dependent proteins Streptomyces virginiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 2-methylpropanoyl-CoA pH 7.4, 30ºC Streptomyces virginiae
0.057
-
 butanoyl-CoA pH 7.4, 30ºC Streptomyces virginiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14300
-
 alpha2beta2, IcmA2IcmB2, 2 * 62500 + 2 * 14300, gel filtration, IcmB provides the cobalamin-binding domain Streptomyces virginiae
62500
-
 alpha2beta2, IcmA2IcmB2, 2 * 62500 + 2 * 14300, gel filtration, IcmB provides the cobalamin-binding domain Streptomyces virginiae
153000
-
 gel filtration Streptomyces virginiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methylpropanoyl-CoA Streptomyces virginiae valine and fatty acid catabolism butanoyl-CoA
-
 r
2-methylpropanoyl-CoA Streptomyces virginiae biosynthesis of the antibiotic monesin-A butanoyl-CoA
-
 r
2-methylpropanoyl-CoA Streptomyces virginiae A3823.5 valine and fatty acid catabolism butanoyl-CoA
-
 r
2-methylpropanoyl-CoA Streptomyces virginiae A3823.5 biosynthesis of the antibiotic monesin-A butanoyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Streptomyces virginiae Q9RJ84 A3823.5
-
Streptomyces virginiae A3823.5 Q9RJ84 A3823.5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant IcmB is purified using Q-Sepharose, and two Mono Q column chromatography Streptomyces virginiae

Reaction

Reaction Comment Organism Reaction ID
2-methylpropanoyl-CoA = butanoyl-CoA either a random or ordered sequential mechanism Streptomyces virginiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3
-
 the specific activity varies with the molar ratio of IcmA to IcmB Streptomyces virginiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylpropanoyl-CoA
-
 Streptomyces virginiae butanoyl-CoA
-
 r
2-methylpropanoyl-CoA valine and fatty acid catabolism Streptomyces virginiae butanoyl-CoA
-
 r
2-methylpropanoyl-CoA biosynthesis of the antibiotic monesin-A Streptomyces virginiae butanoyl-CoA
-
 r
2-methylpropanoyl-CoA
-
 Streptomyces virginiae A3823.5 butanoyl-CoA
-
 r
2-methylpropanoyl-CoA valine and fatty acid catabolism Streptomyces virginiae A3823.5 butanoyl-CoA
-
 r
2-methylpropanoyl-CoA biosynthesis of the antibiotic monesin-A Streptomyces virginiae A3823.5 butanoyl-CoA
-
 r

Subunits

Subunits Comment Organism
tetramer alpha2beta2, IcmA2IcmB2, 2 * 62500 + 2 * 14300, gel filtration, IcmB provides the cobalamin-binding domain Streptomyces virginiae

Synonyms

Synonyms Comment Organism
ICM
-
 Streptomyces virginiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 the reaction is incubated at darkness Streptomyces virginiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.2
-
 butanoyl-CoA pH 7.4, 30ºC Streptomyces virginiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 the activity diminishes only slightly at pH 6 and 8 Streptomyces virginiae