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Literature summary for 5.4.99.12 extracted from

  • Lecointe, F.; Namy, O.; Hatin, I.; Simos, G.; Rousset, J-P.; Grosjean, H.
    Lack of pseudouridine 38/39 in the anticodon arm of yeast cytoplasmic tRNA decreases in vivo recoding efficiency (2002), J. Biol. Chem., 277, 30445-30453.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D151A inactive Saccharomyces cerevisiae
D151A inactive mutant Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNA uridine38 Saccharomyces cerevisiae
-
 tRNA pseudouridine38
-
 ?
tRNA uridine39 Saccharomyces cerevisiae
-
 tRNA pseudouridine39
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P31115
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine38
-
 Saccharomyces cerevisiae tRNA pseudouridine38
-
 ?
tRNA uridine39
-
 Saccharomyces cerevisiae tRNA pseudouridine39
-
 ?

Synonyms

Synonyms Comment Organism
PUS3
-
 Saccharomyces cerevisiae
tRNA pseudouridine synthase 3
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction deletion of the PUS3 gene has an effect on the efficiency of the translation process. Reduced readthrough efficiency of each stop codon by natural nonsense suppressor tRNAs Saccharomyces cerevisiae
physiological function deletion of the PUS3 gene, encoding the enzyme that introduces pseudouridines at position 38 or 39 in tRNA, has an effect on the efficiency of the translation process. In the mutant, there is a reduced readthrough efficiency of each stop codon by natural nonsense suppressor tRNAs. This effect is solely due to the absence of pseudouridine 38 or 39 in tRNA. The presence of pseudouridine 38 or 39 in the tRNA anticodon arm enhances misreading of certain codons by natural nonsense tRNAs as well as promotes frameshifting on slippery sequences in yeast Saccharomyces cerevisiae