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Literature summary for 5.4.99.12 extracted from
- Single nucleotide modulation of uridine to pseudouridine rearrangement in transfer RNA catalyzed by pseudouridine synthase I (1996), J. Mol. Recognit., 9, 524-527.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000053 | - |
tRNAHis guanidine36 uridine38 cytidine39 | pH 8.0, 30°C | Escherichia coli | |
| 0.0000456 | - |
tRNAHis uridine38 uridine39 | pH 8.0, 30°C | Escherichia coli | |
| 0.0000804 | - |
tRNAHis uridine38 cytidine39 | pH 8.0, 30°C | Escherichia coli | |
| 0.000669 | - |
tRNAVal2a cytidine36 uridine38 | pH 8.0, 30°C | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | presence of a G36 residue modulates modification at position 38. In addition to local sequence effects, steady-state kinetic analyses suggest the existence of other recognition elements distinct from the immediate vicinity of modification | Escherichia coli | ? | - |
? |  |
| tRNAHis guanidine36 uridine38 cytidine39 | - |
Escherichia coli | RNAHis guanidine36 pseudouridine38 cytidine39 | - |
? | |
| tRNAHis uridine38 cytidine39 | - |
Escherichia coli | RNAHis pseudouridine38 cytidine39 | - |
? | |
| tRNAHis uridine38 uridine39 | - |
Escherichia coli | tRNAHis pseudouridine38 pseudouridine39 | - |
? | |
| tRNAVal2a cytidine36 uridine38 | poor substrate, tRNAVal2a is not a substrate | Escherichia coli | tRNAVal2a cytidine36 pseudouridine38 | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
tRNAHis guanidine36 uridine38 cytidine39 | pH 8.0, 30°C | Escherichia coli | |
| 0.0244 | - |
tRNAVal2a cytidine36 uridine38 | pH 8.0, 30°C | Escherichia coli | |
| 0.0746 | - |
tRNAHis uridine38 cytidine39 | pH 8.0, 30°C | Escherichia coli | |
| 0.144 | - |
tRNAHis uridine38 uridine39 | pH 8.0, 30°C | Escherichia coli |
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