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Literature summary for 5.4.99.11 extracted from

  • Li, L.; Wang, H.; Cheng, H.; Deng, Z.
    Isomaltulose production by yeast surface display of sucrose isomerase from Pantoea dispersa on Yarrowia lipolytica (2017), J. Funct. Foods, 32, 208-217 .
No PubMed abstract available
Search for more literature for 5.4.99.11

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the PdSIase gene into the expression vector pET28a, functional recombinant expression in Yarrowia lipolytica strain CGMCC7326 on the cell surface using the cell wall protein Pir1 as an anchor protein, and recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Pantoea dispersa

Protein Variants

Protein Variants Comment Organism
additional information cloning of the PdSIase gene into the expression vector pET28a, functional recombinant expression in Yarrowia lipolytica strain CGMCC7326 on the cell surface using the cell wall protein Pir1 as an anchor protein. Selection via a hygromycin B resistance gene as a selection marker, method, overview Pantoea dispersa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose Pantoea dispersa
-
 6-O-alpha-D-Glucopyranosyl-D-fructofuranose
-
 r
sucrose Pantoea dispersa UQ68
-
 6-O-alpha-D-Glucopyranosyl-D-fructofuranose
-
 r

Organism

Organism UniProt Comment Textmining
Pantoea dispersa Q6XNK6
-
-
Pantoea dispersa UQ68 Q6XNK6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pantoea dispersa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
-
 Pantoea dispersa 6-O-alpha-D-Glucopyranosyl-D-fructofuranose
-
 r
sucrose
-
 Pantoea dispersa UQ68 6-O-alpha-D-Glucopyranosyl-D-fructofuranose
-
 r

Subunits

Subunits Comment Organism
? x * 66000, recombinant enzyme expressed in Yarrowia lipolytica, SDS-PAGE Pantoea dispersa

Synonyms

Synonyms Comment Organism
PdSIase
-
 Pantoea dispersa
sucrose isomerase
-
 Pantoea dispersa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35 recombinant enzyme Pantoea dispersa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 activity range of recombinant enzyme expressed from Escherichia coli, profile overview Pantoea dispersa
20 55 activity range of recombinant enzyme expressed from Yarrowia lipolytica, profile overview Pantoea dispersa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 recombinant enzyme, completely stable at Pantoea dispersa
52
-
 50% activity remaining of recombinant enzyme expressed from Escherichia coli, inactivation at 65°C, profile overview Pantoea dispersa
60
-
 about 50% activity remaining of recombinant enzyme expressed from Yarrowia lipolytica, inactivation at 70°C, profile overview Pantoea dispersa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7 braod optimum of recombinant enzyme Pantoea dispersa

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 activity range of recombinant enzyme, profile overview Pantoea dispersa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 the yeast strain displaying recombinant enzyme PdSIase is stable throughout relatively broad ranges of pH values and temperatures Pantoea dispersa