Application | Comment | Organism |
---|---|---|
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Erwinia rhapontici |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Serratia plymuthica |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Pantoea dispersa |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Raoultella planticola |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Paraburkholderia acidicola |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Enterobacter sp. FMB-1 |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Klebsiella sp. LX3 |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Rhizobium sp. MX-45 |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Klebsiella pneumoniae |
additional information | in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement | Erwinia sp. D12 |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Erwinia rhapontici |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Serratia plymuthica |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Pantoea dispersa |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Raoultella planticola |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Paraburkholderia acidicola |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Enterobacter sp. FMB-1 |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Klebsiella sp. LX3 |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Rhizobium sp. MX-45 |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Klebsiella pneumoniae |
synthesis | isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose | Erwinia sp. D12 |
Cloned (Comment) | Organism |
---|---|
recombinant enzyme expression | Enterobacter sp. FMB-1 |
recombinant enzyme expression | Erwinia rhapontici |
recombinant enzyme expression | Klebsiella sp. LX3 |
recombinant enzyme expression | Klebsiella pneumoniae |
recombinant enzyme expression from tac vector | Pantoea dispersa |
recombinant enzyme expression from tac vector | Raoultella planticola |
recombinant enzyme expression from tac vector | Erwinia rhapontici |
recombinant enzyme expression, Protaminobacter sucrose isomerase is totally susceptible to inactivation and cannot be solubly expressed with a tac vector either but may fold correctly and is effectively expressed from the L-rhamnose-inducible vector | Serratia plymuthica |
Crystallization (Comment) | Organism |
---|---|
crystal structure determination, PDB ID 1M53 | Klebsiella sp. LX3 |
crystal structure determination, PDB IDs GBD and GBE, with ethylene glycol, citrate, 1,2-ethanediol, and 1-deoxynojirimycin as interacting compounds | Serratia plymuthica |
crystal structure determinations of enzyme mutants F164L and R284C, PDB IDs 4H2C, 4GIN, 4GI6, 4GI8, 4GIA, and 4GI9, interacting compounds are Ca2+, glycerin, D-glucose, and Tris | Rhizobium sp. MX-45 |
crystal structure determinations of native enzyme and enzyme mutants D200A and E254Q, PDB IDs 1ZJB, 2PWH, 1ZJA, 2PWD, 2PWG, 2PWF, and 2PWE, interacting compounds are Ca2+, sucrose, D-glucose, tromethamine, Tris, 1-deoxynojirimycin, and castanospermine | Paraburkholderia acidicola |
Protein Variants | Comment | Organism |
---|---|---|
D200A | enzyme mutant structure analysis | Paraburkholderia acidicola |
E254Q | enzyme mutant structure analysis | Paraburkholderia acidicola |
F164L | enzyme mutant structure analysis | Rhizobium sp. MX-45 |
additional information | immobilization of cells expressing the recombinant wild-type enzyme on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved | Serratia plymuthica |
additional information | immobilization of cells expressing the recombinant wild-type enzyme, and mutant BN 68069 cells, on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved | Erwinia rhapontici |
additional information | immobilization of cells expressing the wild-type enzyme on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved | Serratia plymuthica |
additional information | immobilization of cells expressing the wild-type enzyme on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved | Erwinia rhapontici |
additional information | immobilization of cells expressing the wild-type enzyme on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved | Erwinia sp. D12 |
R284C | enzyme mutant structure analysis | Rhizobium sp. MX-45 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inactivation | Erwinia rhapontici | |
Ag+ | inactivation | Erwinia sp. D12 | |
Ag+ | inactivation | Klebsiella pneumoniae | |
Hg2+ | inactivation | Erwinia rhapontici | |
Hg2+ | inactivation | Erwinia sp. D12 | |
Hg2+ | inactivation | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0192 | - |
sucrose | pH not specified in the publication, 20°C, native enzyme | Paraburkholderia acidicola | |
0.03 | - |
sucrose | pH and temperature not specified in the publication, recombinant enzyme | Paraburkholderia acidicola | |
0.0324 | - |
sucrose | pH and temperature not specified in the publication, recombinant enzyme | Serratia plymuthica | |
0.04 | - |
sucrose | recombinant enzyme, pH not specified in the publication, 35°C | Pantoea dispersa | |
0.0427 | - |
sucrose | recombinant enzyme, pH not specified in the publication, 35°C | Klebsiella pneumoniae | |
0.049 | - |
sucrose | pH not specified in the publication, 50°C, recombinant enzyme | Enterobacter sp. FMB-1 | |
0.0546 | - |
sucrose | recombinant enzyme, pH not specified in the publication, 35°C | Klebsiella sp. LX3 | |
0.065 | - |
sucrose | native enzyme, pH not specified in the publication, 30°C | Serratia plymuthica | |
0.076 | - |
sucrose | recombinant enzyme, pH not specified in the publication, 35°C | Raoultella planticola | |
0.138 | - |
sucrose | pH not specified in the publication, 40°C, native enzyme | Erwinia sp. D12 | |
0.2 | - |
sucrose | recombinant enzyme, pH not specified in the publication, 30°C | Erwinia rhapontici | |
0.222 | - |
sucrose | pH not specified in the publication, 30°C, native enzyme | Erwinia rhapontici | |
0.257 | - |
sucrose | pH not specified in the publication, 30°C, recombinant enzyme | Erwinia rhapontici | |
0.28 | - |
sucrose | pH not specified in the publication, 30°C, native enzyme | Erwinia rhapontici |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Paraburkholderia acidicola | the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase | ? | - |
? | |
additional information | Rhizobium sp. MX-45 | the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase | ? | - |
? | |
additional information | Paraburkholderia acidicola MX-45 | the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase | ? | - |
? | |
sucrose | Erwinia rhapontici | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Serratia plymuthica | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Pantoea dispersa | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Raoultella planticola | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Paraburkholderia acidicola | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Enterobacter sp. FMB-1 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Klebsiella sp. LX3 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Rhizobium sp. MX-45 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Klebsiella pneumoniae | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Erwinia sp. D12 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Erwinia rhapontici DSM 4484 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Klebsiella pneumoniae NK33-98-8 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Raoultella planticola UQ14S | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Erwinia rhapontici NCPPB 1578 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Pantoea dispersa UQ68J | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Serratia plymuthica CBS 574.77 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Erwinia rhapontici wac2928 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Paraburkholderia acidicola MX-45 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Serratia plymuthica ATCC 15928 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | Erwinia rhapontici NX-5 | - |
6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. FMB-1 | B5ABD8 | - |
- |
Erwinia rhapontici | - |
- |
- |
Erwinia rhapontici | D9MPF2 | - |
- |
Erwinia rhapontici | Q6XNK5 | - |
- |
Erwinia rhapontici | Q9AI64 | - |
- |
Erwinia rhapontici DSM 4484 | Q9AI64 | - |
- |
Erwinia rhapontici NCPPB 1578 | - |
- |
- |
Erwinia rhapontici NX-5 | D9MPF2 | - |
- |
Erwinia rhapontici wac2928 | Q6XNK5 | - |
- |
Erwinia sp. D12 | - |
- |
- |
Klebsiella pneumoniae | Q4L2Q1 | - |
- |
Klebsiella pneumoniae NK33-98-8 | Q4L2Q1 | - |
- |
Klebsiella sp. LX3 | Q8KR84 | - |
- |
no activity in Bemisia argentifolii | - |
- |
- |
Pantoea dispersa | Q6XNK6 | - |
- |
Pantoea dispersa UQ68J | Q6XNK6 | - |
- |
Paraburkholderia acidicola | Q2PS28 | - |
- |
Paraburkholderia acidicola MX-45 | Q2PS28 | - |
- |
Raoultella planticola | Q6XKX6 | i.e. Klebsiella planticola | - |
Raoultella planticola UQ14S | Q6XKX6 | i.e. Klebsiella planticola | - |
Rhizobium sp. MX-45 | M1E1F6 | - |
- |
Serratia plymuthica | - |
- |
- |
Serratia plymuthica ATCC 15928 | - |
- |
- |
Serratia plymuthica CBS 574.77 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose | Glu295 plays a role as the general acid catalyst to protonate the oxygen of the glycosidic linkage for substrate hydrolysis, the Odelta2 of Asp241 acts as the nucleophile to attack the C1 of the D-glucosyl and forms a bond with C1 to obtain beta-glucosyl-enzyme intermediate, and the isomaltulose is produced when the O-6 of the hydrolysis product D-fructose nucleophilically binds to the C1 of the D-glucosyl group of the intermediate | Serratia plymuthica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimization of enzyme production from native free cells, culture conditions optimization, overview | Erwinia rhapontici | - |
additional information | optimization of enzyme production from native free cells, culture conditions optimization, overview | Erwinia sp. D12 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.98 | - |
substrate sucrose, pH not specified in the publication, 20°C, native enzyme | Paraburkholderia acidicola |
19.8 | - |
pH not specified in the publication, 40°C, native enzyme | Erwinia sp. D12 |
120 | - |
native enzyme, pH not specified in the publication, 30°C | Serratia plymuthica |
328 | - |
recombinant enzyme, pH not specified in the publication, 35°C | Klebsiella sp. LX3 |
351 | - |
recombinant enzyme, pH not specified in the publication, 35°C | Raoultella planticola |
423 | - |
pH not specified in the publication, 30°C, native enzyme | Erwinia rhapontici |
562 | - |
recombinant enzyme, pH not specified in the publication, 35°C | Pantoea dispersa |
900 | - |
substrate sucrose, pH and temperature not specified in the publication, recombinant enzyme | Paraburkholderia acidicola |
2362 | - |
recombinant enzyme, pH not specified in the publication, 35°C | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase | Paraburkholderia acidicola | ? | - |
? | |
additional information | the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase | Rhizobium sp. MX-45 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Erwinia rhapontici | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Serratia plymuthica | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Pantoea dispersa | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Raoultella planticola | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Paraburkholderia acidicola | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Enterobacter sp. FMB-1 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Klebsiella sp. LX3 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Rhizobium sp. MX-45 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Klebsiella pneumoniae | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Erwinia sp. D12 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Erwinia rhapontici DSM 4484 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Klebsiella pneumoniae NK33-98-8 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Raoultella planticola UQ14S | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Erwinia rhapontici NCPPB 1578 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Pantoea dispersa UQ68J | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Serratia plymuthica CBS 574.77 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Erwinia rhapontici wac2928 | ? | - |
? | |
additional information | the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase | Paraburkholderia acidicola MX-45 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Paraburkholderia acidicola MX-45 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Serratia plymuthica ATCC 15928 | ? | - |
? | |
additional information | the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides | Erwinia rhapontici NX-5 | ? | - |
? | |
sucrose | - |
Erwinia rhapontici | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Serratia plymuthica | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Pantoea dispersa | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Raoultella planticola | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Paraburkholderia acidicola | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Enterobacter sp. FMB-1 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Klebsiella sp. LX3 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Rhizobium sp. MX-45 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Klebsiella pneumoniae | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Erwinia sp. D12 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Erwinia rhapontici DSM 4484 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Klebsiella pneumoniae NK33-98-8 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Raoultella planticola UQ14S | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Erwinia rhapontici NCPPB 1578 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Pantoea dispersa UQ68J | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Serratia plymuthica CBS 574.77 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Erwinia rhapontici wac2928 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Paraburkholderia acidicola MX-45 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Serratia plymuthica ATCC 15928 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r | |
sucrose | - |
Erwinia rhapontici NX-5 | 6-O-alpha-D-Glucopyranosyl-D-fructofuranose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, native enzyme | Erwinia sp. D12 |
? | x * 64300, recombinant enzyme, x * 63000, native enzyme | Paraburkholderia acidicola |
? | x * 65000, native enzyme | Serratia plymuthica |
? | x * 65600, native enzyme, x * 66000, recombinant enzyme | Erwinia rhapontici |
? | x * 69940, recombinant enzyme | Klebsiella sp. LX3 |
? | x * 69980, recombinant enzyme | Klebsiella pneumoniae |
? | x * 70000, recombinant enzyme | Enterobacter sp. FMB-1 |
Synonyms | Comment | Organism |
---|---|---|
Sucrose alpha-glucosyltransferase | - |
Erwinia rhapontici |
Sucrose alpha-glucosyltransferase | - |
Serratia plymuthica |
Sucrose alpha-glucosyltransferase | - |
Pantoea dispersa |
Sucrose alpha-glucosyltransferase | - |
Raoultella planticola |
Sucrose alpha-glucosyltransferase | - |
Paraburkholderia acidicola |
Sucrose alpha-glucosyltransferase | - |
Enterobacter sp. FMB-1 |
Sucrose alpha-glucosyltransferase | - |
Klebsiella sp. LX3 |
Sucrose alpha-glucosyltransferase | - |
Rhizobium sp. MX-45 |
Sucrose alpha-glucosyltransferase | - |
Klebsiella pneumoniae |
Sucrose alpha-glucosyltransferase | - |
Erwinia sp. D12 |
sucrose isomerase | - |
Erwinia rhapontici |
sucrose isomerase | - |
Serratia plymuthica |
sucrose isomerase | - |
Pantoea dispersa |
sucrose isomerase | - |
Raoultella planticola |
sucrose isomerase | - |
Paraburkholderia acidicola |
sucrose isomerase | - |
Enterobacter sp. FMB-1 |
sucrose isomerase | - |
Klebsiella sp. LX3 |
sucrose isomerase | - |
Rhizobium sp. MX-45 |
sucrose isomerase | - |
Klebsiella pneumoniae |
sucrose isomerase | - |
Erwinia sp. D12 |
Trehalulose synthase | - |
Erwinia rhapontici |
Trehalulose synthase | - |
Serratia plymuthica |
Trehalulose synthase | - |
Pantoea dispersa |
Trehalulose synthase | - |
Raoultella planticola |
Trehalulose synthase | - |
Paraburkholderia acidicola |
Trehalulose synthase | - |
Enterobacter sp. FMB-1 |
Trehalulose synthase | - |
Klebsiella sp. LX3 |
Trehalulose synthase | - |
Rhizobium sp. MX-45 |
Trehalulose synthase | - |
Klebsiella pneumoniae |
Trehalulose synthase | - |
Erwinia sp. D12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
native enzyme | Paraburkholderia acidicola |
30 | - |
- |
Erwinia rhapontici |
30 | - |
- |
Serratia plymuthica |
30 | - |
native and recombinant enzyme | Erwinia rhapontici |
35 | - |
- |
Pantoea dispersa |
35 | - |
- |
Raoultella planticola |
35 | - |
- |
Klebsiella sp. LX3 |
40 | - |
- |
Erwinia sp. D12 |
50 | - |
recombinant enzyme | Enterobacter sp. FMB-1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paraburkholderia acidicola | native enzyme | - |
5.4 |
Klebsiella pneumoniae | recombinant enzyme | - |
5.89 |
Erwinia rhapontici | native enzyme | - |
6 |
Enterobacter sp. FMB-1 | recombinant enzyme | - |
6.02 |
Klebsiella sp. LX3 | recombinant enzyme | - |
6.62 |
Serratia plymuthica | - |
- |
9 |
General Information | Comment | Organism |
---|---|---|
additional information | the unique RLDRD motif is located in a loop adjacent to the active site cleft and is determined to play an important role in the isomerization process and in the control of product specificity | Serratia plymuthica |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Erwinia rhapontici |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Serratia plymuthica |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Pantoea dispersa |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Raoultella planticola |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Paraburkholderia acidicola |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Enterobacter sp. FMB-1 |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Klebsiella sp. LX3 |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Rhizobium sp. MX-45 |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Klebsiella pneumoniae |
physiological function | physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview | Erwinia sp. D12 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.19 | - |
sucrose | recombinant enzyme, pH not specified in the publication, 35°C | Raoultella planticola | |
17.9 | - |
sucrose | recombinant enzyme, pH not specified in the publication, 35°C | Pantoea dispersa | |
36 | - |
sucrose | pH and temperature not specified in the publication, recombinant enzyme | Paraburkholderia acidicola | |
1301 | - |
sucrose | pH and temperature not specified in the publication, recombinant enzyme | Serratia plymuthica |