Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression of His6-tagged enzyme in Escherichia coli | Pectobacterium carotovorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68800 | - |
x * 68800, about, sequence calculation | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | Pectobacterium carotovorum | production of trehalulose and isomaltulose in a ratio of 64:36 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
sucrose | Pectobacterium carotovorum KKH 3-1 | production of trehalulose and isomaltulose in a ratio of 64:36 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | S5YEW8 | - |
- |
Pectobacterium carotovorum KKH 3-1 | S5YEW8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickle affinity chromatography and gel filtration | Pectobacterium carotovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | production of trehalulose and isomaltulose in a ratio of 64:36 | Pectobacterium carotovorum | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
sucrose | production of trehalulose and isomaltulose in a ratio of 64:36 at 40°C. The product ratio depends on the reaction temperature, overview. The amount of trehalulose increases from 47.5% to 79.1% as temperature is lowered from 50°C to 30°C | Pectobacterium carotovorum | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
sucrose | production of trehalulose and isomaltulose in a ratio of 64:36 | Pectobacterium carotovorum KKH 3-1 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
sucrose | production of trehalulose and isomaltulose in a ratio of 64:36 at 40°C. The product ratio depends on the reaction temperature, overview. The amount of trehalulose increases from 47.5% to 79.1% as temperature is lowered from 50°C to 30°C | Pectobacterium carotovorum KKH 3-1 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68800, about, sequence calculation | Pectobacterium carotovorum |
Synonyms | Comment | Organism |
---|---|---|
SIase | - |
Pectobacterium carotovorum |
sucrose isomerase | - |
Pectobacterium carotovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pectobacterium carotovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pectobacterium carotovorum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pectobacterium carotovorum | sequence calculation | - |
5.96 |