Protein Variants | Comment | Organism |
---|---|---|
D327N | turnover number for sucrose: 1.4fold decrease, KM-value for sucrose: 1.65fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 43.7% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 41.0% increase | Klebsiella sp. |
D327R | turnover number for sucrose: 2.45fold decrease, KM-value for sucrose: 2.2fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 26.5% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 17.7% increase | Klebsiella sp. |
D329N | turnover number for sucrose: 1.17fold decrease, KM-value for sucrose: 2.9fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 52.4% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 54.7% increase | Klebsiella sp. |
R325D | turnover number for sucrose: 14.5fold decrease, KM-value for sucrose: 1.2fold decrease, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 67% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 43.6% increase | Klebsiella sp. |
R325L | turnover number for sucrose: 14.5fold decrease, KM-value for sucrose: 1.1fold decrease, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 54.3% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 31.3% increase | Klebsiella sp. |
R328D | turnover number for sucrose: 11.9fold decrease, KM-value for sucrose: 2fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 65.6% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 61.2% increase | Klebsiella sp. |
R328L | turnover number for sucrose: 14.5fold decrease, KM-value for sucrose: 5fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 51.9% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 50.3% increase | Klebsiella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44 | - |
sucrose | mutant enzyme R325D, pH 6.0, 35°C | Klebsiella sp. | |
49.1 | - |
sucrose | mutant enzyme R325L, pH 6.0, 35°C | Klebsiella sp. | |
54.3 | - |
sucrose | wild-type enzyme, pH 6.0, 35°C | Klebsiella sp. | |
89.9 | - |
sucrose | mutant enzyme D327N, pH 6.0, 35°C | Klebsiella sp. | |
108.9 | - |
sucrose | mutant enzyme R328D, pH 6.0, 35°C | Klebsiella sp. | |
121.7 | - |
sucrose | mutant enzyme D327R, pH 6.0, 35°C | Klebsiella sp. | |
157.7 | - |
sucrose | mutant enzyme R329N, pH 6.0, 35°C | Klebsiella sp. | |
195.5 | - |
sucrose | mutant enzyme R328L, pH 6.0, 35°C | Klebsiella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | - |
Klebsiella sp. | alpha-D-glucopyranosyl-1,6-D-fructofuranose + alpha-D-glucopyranosyl-1,1-D-fructofuranose | wild-type enzyme: 82.8% alpha-D-glucopyranosyl-1,6-D-fructofuranose and 12.1% alpha-D-glucopyranosyl-1,1-D-fructofuranose | ? |
Synonyms | Comment | Organism |
---|---|---|
isomaltulose synthase | - |
Klebsiella sp. |
PalI | - |
Klebsiella sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
720 | - |
sucrose | mutant enzyme R325D and R325L, pH 6.0, 35°C | Klebsiella sp. | |
876 | - |
sucrose | mutant enzyme R328D, pH 6.0, 35°C | Klebsiella sp. | |
2070 | - |
sucrose | mutant enzyme R328L, pH 6.0, 35°C | Klebsiella sp. | |
4260 | - |
sucrose | mutant enzyme D327R, pH 6.0, 35°C | Klebsiella sp. | |
7368 | - |
sucrose | mutant enzyme D327N, pH 6.0, 35°C | Klebsiella sp. | |
7370 | - |
sucrose | mutant enzyme D327N, pH 6.0, 35°C | Klebsiella sp. | |
8898 | - |
sucrose | mutant enzyme D329L, pH 6.0, 35°C | Klebsiella sp. | |
8900 | - |
sucrose | mutant enzyme D329L, pH 6.0, 35°C | Klebsiella sp. | |
10400 | - |
sucrose | wild-type enzyme, pH 6.0, 35°C | Klebsiella sp. | |
10430 | - |
sucrose | wild-type enzyme, pH 6.0, 35°C | Klebsiella sp. |