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Literature summary for 5.4.99.1 extracted from

  • Holloway, D.E.; Harding, S.E.; Marsh, E.N.G.
    Adenosylcobalamin-dependent glutamate mutase: properties of a fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit (1996), Biochem. J., 320, 825-830.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Benzimidazolribofuranosyl-adenosylcobinamide can serve as cofactor, Km-value in reaction with L-Glu, fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit: 0.0048 mM. Km-value in reaction with L-Glu, wild-type enzyme: 0.0005 mM Clostridium tetanomorphum

Protein Variants

Protein Variants Comment Organism
additional information fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit Clostridium tetanomorphum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
L-Glu reaction with benzimidazolribofuranosyl-adenosylcobinamide, wild-type enzyme and fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit Clostridium tetanomorphum

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit Clostridium tetanomorphum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Glu
-
Clostridium tetanomorphum threo-3-Methylaspartate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.65
-
L-Glu reaction of L-Glu with benzimidazolribofuranosyl-adenosylcobinamide, fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit Clostridium tetanomorphum
20
-
L-Glu reaction of L-Glu with benzimidazolribofuranosyl-adenosylcobinamide, wild-type enzyme Clostridium tetanomorphum

Cofactor

Cofactor Comment Organism Structure
Cobalamin dependent on Clostridium tetanomorphum