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Literature summary for 5.4.99.1 extracted from

  • Buckel, W.; Golding, B.T.
    Glutamate and 2-methyleneglutarate mutase: from microbial curiosities to paradigms for coenzyme B12-dependent enzymes (1996), Chem. Soc. Rev., 25, 329-337.
No PubMed abstract available
Search for more literature for 5.4.99.1

Cloned(Commentary)

Cloned (Comment) Organism
polypeptide chains sigma and epsilon expressed in Escherichia coli Clostridium cochlearium
polypeptide chains sigma and epsilon expressed in Escherichia coli Clostridium tetanomorphum

Organism

Organism UniProt Comment Textmining
Clostridium cochlearium
-
-
-
Clostridium tetanomorphum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-threo-3-methylaspartate = L-glutamate requires Co(II) for coordiation of the acrylate, in order to enable the addition of the radical fragment to the alpha-carbon of this intermediate, leading to the branched product Clostridium cochlearium
a
L-threo-3-methylaspartate = L-glutamate requires Co(II) for coordiation of the acrylate, in order to enable the addition of the radical fragment to the alpha-carbon of this intermediate, leading to the branched product Clostridium tetanomorphum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Glu
-
 Clostridium cochlearium threo-3-Methylaspartate
-
 ?
L-Glu
-
 Clostridium tetanomorphum threo-3-Methylaspartate
-
 ?

Subunits

Subunits Comment Organism
? enzyme is composed of two components: E, a dimer, epsilon2, and S, a monomer, sigma = 14700 Clostridium cochlearium
? enzyme is composed of two components: E, a dimer, epsilon2, and S, a monomer, sigma = 14700 Clostridium tetanomorphum

Cofactor

Cofactor Comment Organism Structure
Cobalamin dependent on Clostridium cochlearium
Cobalamin dependent on Clostridium tetanomorphum
Cobalamin requires Co(II) for coordination of the acrylate, in order to enable the addition of the radical fragment to the alpha-carbon of this intermediate, leading to the branched product Clostridium cochlearium
Cobalamin requires Co(II) for coordination of the acrylate, in order to enable the addition of the radical fragment to the alpha-carbon of this intermediate, leading to the branched product Clostridium tetanomorphum