BRENDA - Enzyme Database
show all sequences of 5.4.99.1

Coenzyme B12-dependent mutases causing carbon chain rearrangements

Barker, H.A.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 6, 509-537 (1972)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
3-methylaspartate
-
Clostridium cochlearium
0.5
-
3-methylaspartate
-
Clostridium tetanomorphum
1.5
-
L-Glu
-
Clostridium cochlearium
1.5
-
L-Glu
-
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Glu
Rhodobacter sphaeroides
-
?
-
-
-
Glu
Rhodospirillum rubrum
-
?
-
-
-
Glu
Clostridium cochlearium
-
?
-
-
-
Glu
Clostridium tetanomorphum
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium cochlearium
-
-
-
Clostridium tetanomorphum
-
-
-
Rhodobacter sphaeroides
-
-
-
Rhodospirillum rubrum
-
-
-
Purification (Commentary)
Commentary
Organism
purification of component E and component S
Clostridium tetanomorphum
Reaction
Reaction
Commentary
Organism
L-threo-3-methylaspartate = L-glutamate
mechanism
Clostridium tetanomorphum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Glu
-
3453
Rhodobacter sphaeroides
?
-
-
-
-
Glu
-
3453
Rhodospirillum rubrum
?
-
-
-
-
Glu
-
3453
Clostridium cochlearium
?
-
-
-
-
Glu
-
3453
Clostridium tetanomorphum
?
-
-
-
-
L-Glu
-
3453
Clostridium cochlearium
threo-3-Methylaspartate
-
3453
Clostridium cochlearium
-
L-Glu
r
3453
Rhodobacter sphaeroides
threo-3-Methylaspartate
-
3453
Rhodobacter sphaeroides
-
L-Glu
r
3453
Rhodospirillum rubrum
threo-3-Methylaspartate
-
3453
Rhodospirillum rubrum
-
L-Glu
r
3453
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3453
Clostridium tetanomorphum
-
L-Glu
equilibrium favours glutamate formation
3453
Rhodobacter sphaeroides
threo-3-Methylaspartate
-
3453
Rhodobacter sphaeroides
-
L-Glu
equilibrium favours glutamate formation
3453
Rhodospirillum rubrum
threo-3-Methylaspartate
-
3453
Rhodospirillum rubrum
-
L-Glu
equilibrium favours glutamate formation
3453
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3453
Clostridium tetanomorphum
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
maximal activity in a 30 min incubation period
Clostridium tetanomorphum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
rapidly inactivated
Clostridium tetanomorphum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Clostridium tetanomorphum
Cofactor
Cofactor
Commentary
Organism
Structure
Cobalamin
-
Clostridium cochlearium
Cobalamin
and at least seven analogs containing bases other than dimethylbenzimidazolyl can serve as coenzymes
Clostridium tetanomorphum
Cobalamin
-
Rhodobacter sphaeroides
Cobalamin
-
Rhodospirillum rubrum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Cobalamin
-
Clostridium cochlearium
Cobalamin
and at least seven analogs containing bases other than dimethylbenzimidazolyl can serve as coenzymes
Clostridium tetanomorphum
Cobalamin
-
Rhodobacter sphaeroides
Cobalamin
-
Rhodospirillum rubrum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
3-methylaspartate
-
Clostridium cochlearium
0.5
-
3-methylaspartate
-
Clostridium tetanomorphum
1.5
-
L-Glu
-
Clostridium cochlearium
1.5
-
L-Glu
-
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Glu
Rhodobacter sphaeroides
-
?
-
-
-
Glu
Rhodospirillum rubrum
-
?
-
-
-
Glu
Clostridium cochlearium
-
?
-
-
-
Glu
Clostridium tetanomorphum
-
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
purification of component E and component S
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Glu
-
3453
Rhodobacter sphaeroides
?
-
-
-
-
Glu
-
3453
Rhodospirillum rubrum
?
-
-
-
-
Glu
-
3453
Clostridium cochlearium
?
-
-
-
-
Glu
-
3453
Clostridium tetanomorphum
?
-
-
-
-
L-Glu
-
3453
Clostridium cochlearium
threo-3-Methylaspartate
-
3453
Clostridium cochlearium
-
L-Glu
r
3453
Rhodobacter sphaeroides
threo-3-Methylaspartate
-
3453
Rhodobacter sphaeroides
-
L-Glu
r
3453
Rhodospirillum rubrum
threo-3-Methylaspartate
-
3453
Rhodospirillum rubrum
-
L-Glu
r
3453
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3453
Clostridium tetanomorphum
-
L-Glu
equilibrium favours glutamate formation
3453
Rhodobacter sphaeroides
threo-3-Methylaspartate
-
3453
Rhodobacter sphaeroides
-
L-Glu
equilibrium favours glutamate formation
3453
Rhodospirillum rubrum
threo-3-Methylaspartate
-
3453
Rhodospirillum rubrum
-
L-Glu
equilibrium favours glutamate formation
3453
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3453
Clostridium tetanomorphum
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
maximal activity in a 30 min incubation period
Clostridium tetanomorphum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
rapidly inactivated
Clostridium tetanomorphum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Clostridium tetanomorphum
Other publictions for EC 5.4.99.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748550
Wang
Production of mesaconate in E ...
Clostridium tetanomorphum, Clostridium tetanomorphum ATCC 15920, no activity in Escherichia coli
Metab. Eng.
30
190-196
2015
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1
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1
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2
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5
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1
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2
1
1
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1
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2
1
1
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1
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1
1
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728191
Rommel
Role of tunneling in the enzym ...
Clostridium cochlearium
J. Phys. Chem. B
116
13682-13689
2012
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714983
Ramezani
Glutamate racemization and cat ...
Fusobacterium varium
FEBS J.
278
2540-2551
2011
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2
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4
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4
1
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1
1
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727661
Rommel
The fragmentation-recombinatio ...
Clostridium cochlearium
J. Am. Chem. Soc.
133
10195-10203
2011
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1
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1
1
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702376
Yoon
Hydrogen tunneling in adenosyl ...
Homo sapiens
Biochemistry
49
3168-3173
2010
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1
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1
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716014
Kozlowski
Theoretical analysis of the di ...
Clostridium cochlearium
J. Phys. Chem. B
114
5928-5939
2010
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3
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2
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716116
Biedendieck
Metabolic engineering of cobal ...
Bacillus megaterium
Microb. Biotechnol.
3
24-37
2010
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1
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1
1
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692318
Chen
Interactions between coenzyme ...
Clostridium tetanomorphum
FEBS J.
275
5960-5968
2008
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1
1
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677821
Patwardhan
Changes in the free energy pro ...
Clostridium cochlearium
Arch. Biochem. Biophys.
461
194-199
2007
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2
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678315
Cheng
Evidence for coupled motion an ...
Clostridium cochlearium
Biochemistry
46
883-889
2007
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680366
Sandala
Toward an improved understandi ...
Clostridium cochlearium
J. Am. Chem. Soc.
129
1623-1633
2007
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678147
Yoon
Reaction of adenosylcobalamin- ...
Clostridium cochlearium
Biochemistry
45
11650-11657
2006
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679538
Weng
-
Optimization of the overexpres ...
Clostridium tetanomorphum
Enzyme Microb. Technol.
38
465-469
2006
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661176
Brooks
Electronic structure studies o ...
Clostridium cochlearium
Biochemistry
44
15167-15181
2005
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661188
Cheng
Isotope effects for deuterium ...
Clostridium cochlearium
Biochemistry
44
2686-2691
2005
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662705
Sension
Time-resolved measurements of ...
Clostridium cochlearium
J. Phys. Chem. B
109
18146-18152
2005
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662763
Li
Improvement of nikkomycin prod ...
Streptomyces ansochromogenes
Metab. Eng.
7
165-173
2005
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650247
Xia
Role of arg100 in the active s ...
Clostridium cochlearium
Biochemistry
43
3238-3245
2004
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Pre-steady-state measurement o ...
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Photolysis and recombination o ...
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A novel reaction between adeno ...
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The role of the active site gl ...
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A protein pre-organized to tra ...
Clostridium tetanomorphum
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The B(12)-binding subunit of g ...
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649913
Roymoulik
Rearrangement of L-2-hydroxygl ...
Clostridium cochlearium
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Chih
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Mechanism of glutamate mutase: ...
Clostridium cochlearium
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Structure and dynamics of the ...
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651916
Roymoulik
The reaction of the substrate ...
Clostridium cochlearium
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653936
Reitzer
Glutamate mutase from Clostrid ...
Clostridium cochlearium
Structure
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891-902
1999
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3446
Bothe
Identification of the 4-glutam ...
Clostridium cochlearium
Biochemistry
37
4105-4113
1998
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1
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1
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3462
Reitzer
Crystallization and preliminar ...
Clostridium cochlearium
Acta Crystallogr. Sect. D
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1998
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1
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1
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3464
Tollinger
How a protein prepares for B12 ...
Clostridium tetanomorphum
Structure
15
1021-1033
1998
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4
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1
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1
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1
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3455
Chen
Adenosylcobalamin-dependent gl ...
Clostridium cochlearium
Biochemistry
36
14939-14945
1997
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2
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3457
Buckel
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Clostridium cochlearium, Clostridium tetanomorphum
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329-337
1996
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3458
Holloway
Adenosylcobalamin-dependent gl ...
Clostridium tetanomorphum
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1996
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1
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2
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3459
Holloway
Carboxymethylation of MutS-cys ...
Clostridium tetanomorphum
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1996
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3
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3454
Marsh
Tritium isotope effects in ade ...
Clostridium tetanomorphum
Biochemistry
34
7542-7547
1995
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3456
Hartzoulakis
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The mechanism of glutamate mut ...
Clostridium tetanomorphum
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1994
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3460
Holloway
Adenosylcobalamin-dependent gl ...
Clostridium tetanomorphum
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1994
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1
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1
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2
1
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3463
Zelder
Characterization of the coenzy ...
Clostridium cochlearium
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226
577-585
1994
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1
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2
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2
1
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3461
Leutbecher
Glutamate mutase from Clostrid ...
Clostridium cochlearium
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1992
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1
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1
1
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3452
Barker
beta-Methylaspartate-glutamate ...
Clostridium tetanomorphum
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1985
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3451
Switzer
-
Glutamate mutase ...
Acetoanaerobium sticklandii, Clostridium cochlearium, Clostridium saccharobutyricum, Clostridium sp., Clostridium sp. SB4, Clostridium tetani, Clostridium tetanomorphum, no activity in Acidaminococcus fermentans, no activity in Clostridium microsporum, no activity in Fusobacterium fusiforme, no activity in Fusobacterium nucleatum, no activity in Micrococcus aerogenes, Rhodobacter sphaeroides, Rhodospirillum rubrum
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9
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19
2
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1
1
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3449
Ohmori
-
Metabolism of glutamate in pur ...
Rhodobacter sphaeroides, Rhodospirillum rubrum
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359-365
1974
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2
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2
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2
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-
-
-
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4
-
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3448
Eagar
Mechanism of action of coenzym ...
Clostridium tetanomorphum
Biochemistry
11
253-264
1972
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1
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3453
Barker
-
Coenzyme B12-dependent mutases ...
Clostridium cochlearium, Clostridium tetanomorphum, Rhodobacter sphaeroides, Rhodospirillum rubrum
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
6
509-537
1972
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4
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4
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4
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1
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11
-
1
-
1
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1
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-
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-
-
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3447
Ohmori
Vitamin B12 dependent glutamat ...
Rhodobacter sphaeroides, Rhodospirillum rubrum
Biochem. Biophys. Res. Commun.
43
156-162
1971
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2
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3
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2
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2
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2
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2
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2
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3450
Barker
-
Glutamate mutase ...
Clostridium tetanomorphum
Methods Enzymol.
13
319-330
1969
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1
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1
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