BRENDA - Enzyme Database
show all sequences of 5.4.99.1

Identification of the 4-glutamyl radical as an intermediate in the carbon skeleton rearrangement catalyzed by coenzyme B12-dependent glutamate mutase from Clostridium cochlearium

Bothe, H.; Darley, D.J.; Albracht, S.P.; Gerfen, G.J.; Golding, B.T.; Buckel, W.; Biochemistry 37, 4105-4113 (1998)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium cochlearium
-
-
-
Reaction
Reaction
Commentary
Organism
L-threo-3-methylaspartate = L-glutamate
the 4-glutamyl radical is an intermediate in the carbon skeleton rearrangement catalyzed the enzyme; the reaction is initiated through hydrogen atom abstraction from C-4 of Glu by 5'-deoxyadenosyl radical which is derived by homolysis of the Co-C sigma-bond of coenzyme B12
Clostridium cochlearium
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Glu
-
3446
Clostridium cochlearium
threo-3-Methylaspartate
-
3446
Clostridium cochlearium
-
Cofactor
Cofactor
Commentary
Organism
Structure
Cobalamin
dependent on
Clostridium cochlearium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Cobalamin
dependent on
Clostridium cochlearium
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Glu
-
3446
Clostridium cochlearium
threo-3-Methylaspartate
-
3446
Clostridium cochlearium
-
Other publictions for EC 5.4.99.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748550
Wang
Production of mesaconate in E ...
Clostridium tetanomorphum, Clostridium tetanomorphum ATCC 15920, no activity in Escherichia coli
Metab. Eng.
30
190-196
2015
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728191
Rommel
Role of tunneling in the enzym ...
Clostridium cochlearium
J. Phys. Chem. B
116
13682-13689
2012
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714983
Ramezani
Glutamate racemization and cat ...
Fusobacterium varium
FEBS J.
278
2540-2551
2011
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727661
Rommel
The fragmentation-recombinatio ...
Clostridium cochlearium
J. Am. Chem. Soc.
133
10195-10203
2011
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1
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702376
Yoon
Hydrogen tunneling in adenosyl ...
Homo sapiens
Biochemistry
49
3168-3173
2010
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716014
Kozlowski
Theoretical analysis of the di ...
Clostridium cochlearium
J. Phys. Chem. B
114
5928-5939
2010
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716116
Biedendieck
Metabolic engineering of cobal ...
Bacillus megaterium
Microb. Biotechnol.
3
24-37
2010
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692318
Chen
Interactions between coenzyme ...
Clostridium tetanomorphum
FEBS J.
275
5960-5968
2008
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677821
Patwardhan
Changes in the free energy pro ...
Clostridium cochlearium
Arch. Biochem. Biophys.
461
194-199
2007
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678315
Cheng
Evidence for coupled motion an ...
Clostridium cochlearium
Biochemistry
46
883-889
2007
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680366
Sandala
Toward an improved understandi ...
Clostridium cochlearium
J. Am. Chem. Soc.
129
1623-1633
2007
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678147
Yoon
Reaction of adenosylcobalamin- ...
Clostridium cochlearium
Biochemistry
45
11650-11657
2006
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679538
Weng
-
Optimization of the overexpres ...
Clostridium tetanomorphum
Enzyme Microb. Technol.
38
465-469
2006
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1
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661176
Brooks
Electronic structure studies o ...
Clostridium cochlearium
Biochemistry
44
15167-15181
2005
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661188
Cheng
Isotope effects for deuterium ...
Clostridium cochlearium
Biochemistry
44
2686-2691
2005
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662705
Sension
Time-resolved measurements of ...
Clostridium cochlearium
J. Phys. Chem. B
109
18146-18152
2005
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662763
Li
Improvement of nikkomycin prod ...
Streptomyces ansochromogenes
Metab. Eng.
7
165-173
2005
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650247
Xia
Role of arg100 in the active s ...
Clostridium cochlearium
Biochemistry
43
3238-3245
2004
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661116
Cheng
Pre-steady-state measurement o ...
Clostridium cochlearium
Biochemistry
43
2155-2158
2004
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661930
Sension
Photolysis and recombination o ...
Clostridium cochlearium
J. Am. Chem. Soc.
126
1598-1599
2004
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650138
Huhta
A novel reaction between adeno ...
Clostridium cochlearium
Biochemistry
41
3200-3206
2002
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650929
Gruber
Coenzyme B(12) dependent gluta ...
Clostridium cochlearium
Curr. Opin. Chem. Biol.
6
598-603
2002
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650824
Madhavapeddi
The role of the active site gl ...
Clostridium cochlearium
Chem. Biol.
8
1143-1149
2001
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6
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650853
Hoffmann
A protein pre-organized to tra ...
Clostridium tetanomorphum
ChemBioChem
2
643-655
2001
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652857
Tollinger
The B(12)-binding subunit of g ...
Clostridium tetanomorphum
J. Mol. Biol.
309
777-791
2001
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649913
Roymoulik
Rearrangement of L-2-hydroxygl ...
Clostridium cochlearium
Biochemistry
39
10340-10346
2000
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1
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651566
Chih
-
Mechanism of glutamate mutase: ...
Clostridium cochlearium
J. Am. Chem. Soc.
122
10732-10733
2000
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1
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651053
Hoffmann
Structure and dynamics of the ...
Clostridium cochlearium
Eur. J. Biochem.
263
178-188
1999
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4
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1
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651916
Roymoulik
The reaction of the substrate ...
Clostridium cochlearium
J. Biol. Chem.
274
11619-11622
1999
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1
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653936
Reitzer
Glutamate mutase from Clostrid ...
Clostridium cochlearium
Structure
7
891-902
1999
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1
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3446
Bothe
Identification of the 4-glutam ...
Clostridium cochlearium
Biochemistry
37
4105-4113
1998
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3
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1
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1
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-
3462
Reitzer
Crystallization and preliminar ...
Clostridium cochlearium
Acta Crystallogr. Sect. D
54
1039-1042
1998
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-
1
1
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1
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3
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1
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1
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1
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1
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1
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1
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1
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3464
Tollinger
How a protein prepares for B12 ...
Clostridium tetanomorphum
Structure
15
1021-1033
1998
-
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1
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2
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4
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1
1
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1
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1
1
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2
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1
1
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3455
Chen
Adenosylcobalamin-dependent gl ...
Clostridium cochlearium
Biochemistry
36
14939-14945
1997
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1
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2
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1
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1
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2
2
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1
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1
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1
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2
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1
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2
2
-
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3457
Buckel
-
Glutamate and 2-methylenegluta ...
Clostridium cochlearium, Clostridium tetanomorphum
Chem. Soc. Rev.
25
329-337
1996
-
-
2
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2
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2
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2
2
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2
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2
2
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-
2
2
-
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3458
Holloway
Adenosylcobalamin-dependent gl ...
Clostridium tetanomorphum
Biochem. J.
320
825-830
1996
1
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1
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1
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2
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1
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1
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2
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1
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1
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1
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1
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1
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1
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-
1
-
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-
-
2
-
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-
-
-
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3459
Holloway
Carboxymethylation of MutS-cys ...
Clostridium tetanomorphum
J. Biol. Chem.
271
29121-29125
1996
-
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3
-
1
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1
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1
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1
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1
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3
-
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1
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1
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-
-
3454
Marsh
Tritium isotope effects in ade ...
Clostridium tetanomorphum
Biochemistry
34
7542-7547
1995
-
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1
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1
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1
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1
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-
-
3456
Hartzoulakis
-
The mechanism of glutamate mut ...
Clostridium tetanomorphum
Proc. Indian Acad. Sci. Chem. Sci.
106
1165-1176
1994
-
-
-
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10
-
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1
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1
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1
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1
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1
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-
-
10
-
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-
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-
-
-
1
-
-
-
-
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-
-
-
-
-
-
3460
Holloway
Adenosylcobalamin-dependent gl ...
Clostridium tetanomorphum
J. Biol. Chem.
269
20425-20430
1994
-
-
1
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-
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5
-
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1
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1
2
1
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1
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1
-
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1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3463
Zelder
Characterization of the coenzy ...
Clostridium cochlearium
Eur. J. Biochem.
226
577-585
1994
-
-
1
-
-
-
1
-
-
-
-
1
-
4
-
-
1
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3461
Leutbecher
Glutamate mutase from Clostrid ...
Clostridium cochlearium
Eur. J. Biochem.
205
759-765
1992
-
-
-
-
-
-
5
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3452
Barker
beta-Methylaspartate-glutamate ...
Clostridium tetanomorphum
Methods Enzymol.
113
121-133
1985
1
-
-
-
-
-
1
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
1
1
1
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
2
-
-
-
1
-
-
-
1
-
-
-
-
2
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
3451
Switzer
-
Glutamate mutase ...
Acetoanaerobium sticklandii, Clostridium cochlearium, Clostridium saccharobutyricum, Clostridium sp., Clostridium sp. SB4, Clostridium tetani, Clostridium tetanomorphum, no activity in Acidaminococcus fermentans, no activity in Clostridium microsporum, no activity in Fusobacterium fusiforme, no activity in Fusobacterium nucleatum, no activity in Micrococcus aerogenes, Rhodobacter sphaeroides, Rhodospirillum rubrum
B12 (Dolphin, D. ed. ) Wiley, New York
2
289-305
1982
1
-
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-
-
-
-
5
-
-
-
9
-
25
-
-
1
-
-
-
-
-
19
2
-
-
-
-
1
1
-
4
-
-
-
1
-
-
4
-
-
-
-
-
-
5
-
-
-
9
-
-
-
1
-
-
-
-
19
2
-
-
-
-
1
1
-
-
-
-
-
-
-
-
3449
Ohmori
-
Metabolism of glutamate in pur ...
Rhodobacter sphaeroides, Rhodospirillum rubrum
Agric. Biol. Chem.
38
359-365
1974
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3448
Eagar
Mechanism of action of coenzym ...
Clostridium tetanomorphum
Biochemistry
11
253-264
1972
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
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-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3453
Barker
-
Coenzyme B12-dependent mutases ...
Clostridium cochlearium, Clostridium tetanomorphum, Rhodobacter sphaeroides, Rhodospirillum rubrum
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
6
509-537
1972
-
-
-
-
-
-
-
4
-
-
-
4
-
4
-
-
1
1
-
-
-
-
11
-
1
-
1
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
4
-
-
-
1
-
-
-
-
11
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
3447
Ohmori
Vitamin B12 dependent glutamat ...
Rhodobacter sphaeroides, Rhodospirillum rubrum
Biochem. Biophys. Res. Commun.
43
156-162
1971
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3450
Barker
-
Glutamate mutase ...
Clostridium tetanomorphum
Methods Enzymol.
13
319-330
1969
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
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1
1
3
1
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
3
1
-
-
1
-
-
-
-
-
-
-
-
-