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Literature summary for 5.4.4.2 extracted from

  • Schaaf, P.M.M.; Heide, L.E.; Leistner, E.W.; Tani, Y.; El-Olemy, M.M.
    Immobilization of isochorismate hydroxymutase. Comparison of native versus immobilized enzyme (1993), J. Nat. Prod., 56, 1304-1312.
    View publication on PubMed
Search for more literature for 5.4.4.2

General Stability

General Stability Organism
stability of the enzyme is greatly increased by immobilization Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 isochorismate enzyme immobilized on alkylamine glass Flavobacterium sp.
0.171
-
 isochorismate enzyme immobilized on CNBr-Sepharose Flavobacterium sp.
0.254
-
 isochorismate soluble enzyme Flavobacterium sp.
0.27
-
 chorismate enzyme immobilized on CNBr-Sepharose Flavobacterium sp.
0.313
-
 chorismate enzyme immobilized on alkylamine glass Flavobacterium sp.
0.35
-
 chorismate soluble enzyme Flavobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement Flavobacterium sp.
Mg2+ optimal concentration for soluble enzyme: 5-20 mM. Optimal concentration for enzyme immobilized on CNBr-Sepharose or alkylamine glass: 2.5-5 mM. Activity of the soluble enzyme without Mg2+ is 5.5% of the activity without Mg2+ Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
 K3-15
-
Flavobacterium sp. K3-15
-
 K3-15
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate r Flavobacterium sp. Isochorismate
-
 ?
Chorismate r Flavobacterium sp. K3-15 Isochorismate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
 soluble enzyme Flavobacterium sp.
60
-
 enzyme immobilized on CNBr-Sepharose or alkylamine glass Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 half-life of the enzyme immobilized on alkylamine glass: 210 days. Half-life of enzyme immobilized on CNBr Sepharose: 110 days Flavobacterium sp.
37
-
 40 h, immobilized enzyme, stable Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.5 enzyme immobilized on alkylamine glass Flavobacterium sp.
7.6 8.2 enzyme immobilized on CNBr-Sepharose Flavobacterium sp.
8
-
 soluble enzyme Flavobacterium sp.