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Literature summary for 5.4.3.8 extracted from

  • Orriss, G.L.; Patel, T.R.; Sorensen, J.; Stetefeld, J.
    Absence of a catalytic water confers resistance to the neurotoxin gabaculine (2010), FASEB J., 24, 404-414.
    View publication on PubMed
Search for more literature for 5.4.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Synechococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type enzyme and mutant enzyme M248I are crystallized by the vapor diffusion method, using 11.5% (w/v) PEG 8.000 and 150 mM magnesium acetate, pH 6.8 Synechococcus sp.

Protein Variants

Protein Variants Comment Organism
M248I the M248I point mutation confers about 100fold increased gabaculine resistance to GSAM Synechococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
gabaculine potent inhibitor Synechococcus sp.

Organism

Organism UniProt Comment Textmining
Synechococcus sp. P24630 strain PCC 6301
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0022
-
 mutant enzyme M248I Synechococcus sp.
0.0123
-
 wild type enzyme Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-4-amino-5-oxopentanoate
-
 Synechococcus sp. 5-aminolevulinate
-
 ?

Synonyms

Synonyms Comment Organism
glutamate-1-semialdehyde aminomutase
-
 Synechococcus sp.
GSAM
-
 Synechococcus sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Synechococcus sp.
pyridoxamine 5'-phosphate dependent on Synechococcus sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0028
-
 wild type enzyme Synechococcus sp. gabaculine
0.32
-
 mutant enzyme M248I Synechococcus sp. gabaculine