Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces globisporus |
Protein Variants | Comment | Organism |
---|---|---|
S153A | 1.5% of wild-type activity | Streptomyces globisporus |
S153C | 5% of wild-type activity | Streptomyces globisporus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | weak inhibition | Streptomyces globisporus | |
Cu2+ | strong inhibition | Streptomyces globisporus | |
Zn2+ | weak inhibition | Streptomyces globisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
L-tyrosine | lyase activity | Streptomyces globisporus | |
0.028 | - |
L-tyrosine | - |
Streptomyces globisporus | |
1.54 | - |
L-3,4-dihydroxyphenylalanine | - |
Streptomyces globisporus | |
6.1 | - |
L-3-chlorotyrosine | - |
Streptomyces globisporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58100 | - |
x * 58100, SDS-PAGE | Streptomyces globisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine | Streptomyces globisporus | involved in the biosynthesis of the enediyne antitumor antibiotic C-1027 | 3-amino-3-(4-hydroxyphenyl)propanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces globisporus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant tyrosine aminomutase | Streptomyces globisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-amino-3-(4-hydroxyphenyl)propanoate | beta-tyrosine racemase activity | Streptomyces globisporus | (3R)-amino-3-(4-hydroxyphenyl)propanoate | - |
r | |
L-3,4-dihydroxyphenylalanine | - |
Streptomyces globisporus | 3-amino-3-(3,4-dihydroxyphenyl)propanoate | - |
? | |
L-3-chlorotyrosine | - |
Streptomyces globisporus | 3-amino-3-(3-chloro-4-hydroxyphenyl)propanoate | - |
? | |
L-tyrosine | - |
Streptomyces globisporus | 3-amino-3-(4-hydroxyphenyl)propanoate | - |
r | |
L-tyrosine | involved in the biosynthesis of the enediyne antitumor antibiotic C-1027 | Streptomyces globisporus | 3-amino-3-(4-hydroxyphenyl)propanoate | - |
? | |
L-tyrosine | ammonia lyase activity of tyrosine aminomutase | Streptomyces globisporus | (4-hydroxyphenyl)prop-2-enoate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58100, SDS-PAGE | Streptomyces globisporus |
Synonyms | Comment | Organism |
---|---|---|
SgcC4 | - |
Streptomyces globisporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
L-tyrosine | lyase activity | Streptomyces globisporus | |
0.0048 | - |
L-3-chlorotyrosine | - |
Streptomyces globisporus | |
0.0067 | - |
L-3,4-dihydroxyphenylalanine | - |
Streptomyces globisporus | |
0.01 | - |
L-tyrosine | - |
Streptomyces globisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Streptomyces globisporus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | approx. 50% of maximal activity at pH 7.5 and pH 10.0, respectively | Streptomyces globisporus |