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Literature summary for 5.4.3.3 extracted from
- Identification of a novel pyridoxal 5'-phosphate binding site in adenosylcobalamin-dependent lysine 5,6-aminomutase from Porphyromonas gingivalis (2002), Biochemistry, 41, 8767-8776.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Porphyromonas gingivalis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C235S | 9% of wild-type activity | Porphyromonas gingivalis |
| K144Q | no activity | Porphyromonas gingivalis |
| K23Q | 75% of wild-type activity | Porphyromonas gingivalis |
| K377Q | 0.6% of wild-type activity | Porphyromonas gingivalis |
| K446Q | 25% of wild-type activity | Porphyromonas gingivalis |
| K58Q | 33% of wild-type activity | Porphyromonas gingivalis |
| K90Q | 72% of wild-type activity | Porphyromonas gingivalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-3,6-diaminohexanoate | Porphyromonas gingivalis | - |
(3S,5S)-3,5-diaminohexanoate | - |
? |  |
| D-lysine | Porphyromonas gingivalis | equilibrium constant of 1.2 | 2,5-diaminohexanoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Porphyromonas gingivalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant 5,6-LAM | Porphyromonas gingivalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.31 | - |
- |
Porphyromonas gingivalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-3,6-diaminohexanoate | - |
Porphyromonas gingivalis | (3S,5S)-3,5-diaminohexanoate | - |
? | |
| (3S)-3,6-diaminohexanoate | 5,6-LAM has both beta-lysine 5,6-aminomutase and D-lysine 5,6-aminomutase activity | Porphyromonas gingivalis | (3S,5S)-3,5-diaminohexanoate | - |
? | |
| D-lysine | equilibrium constant of 1.2 | Porphyromonas gingivalis | 2,5-diaminohexanoate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5,6-LAM | - |
Porphyromonas gingivalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| adenosylcobalamin | - |
Porphyromonas gingivalis | |
| pyridoxal 5'-phosphate | - |
Porphyromonas gingivalis | |
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