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Literature summary for 5.4.3.3 extracted from

  • Stadtman, T.C.; Grant, M.A.
    L-beta-Lysine mutase (Clostridium sticklandii) (1971), Methods Enzymol., 17B, 206-211.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2'-deoxyATP or ATP required Acetoanaerobium sticklandii
2-oxobutanoate can partially replace pyruvate, which is required for reaction Acetoanaerobium sticklandii
mercaptan required Acetoanaerobium sticklandii
pyruvate required Acetoanaerobium sticklandii

General Stability

General Stability Organism
highly purified sulfhydryl-enzyme-component is relatively unstable in dilute solutions of low ionic strength. The presence of mercaptans in buffers tends to prevent aggregation. The cobamide enzyme component is stable to a variety of storage conditions even when highly purified Acetoanaerobium sticklandii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Acetoanaerobium sticklandii
Co2+
-
Acetoanaerobium sticklandii
iodoacetamide cobamide enzyme compound is unaffected, sulfhydryl enzyme compounds is rapidly inactivated Acetoanaerobium sticklandii
NH4+
-
Acetoanaerobium sticklandii
Tris and analogs Acetoanaerobium sticklandii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates Acetoanaerobium sticklandii
Mg2+ Mg2+ or Mn2+ required Acetoanaerobium sticklandii
Mn2+ Mg2+ or Mn2+ required Acetoanaerobium sticklandii
Rb+ stimulates Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, gel filtration, sulfhydryl component, x * about approximately 150000, gel filtration, cobamide protein component Acetoanaerobium sticklandii

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,6-Diaminohexanoate r Acetoanaerobium sticklandii 3,5-Diaminohexanoate
-
?

Subunits

Subunits Comment Organism
? x * 60000, gel filtration, sulfhydryl component, x * about approximately 150000, gel filtration, cobamide protein component Acetoanaerobium sticklandii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
10 min, sulfhydryl component is destroyed, cobamide protein component loses about 40% of its activity Acetoanaerobium sticklandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
alpha-(adenyl)-Co-5'-deoxyadenosyl cobamide tightly bound Acetoanaerobium sticklandii
ATP or 2'-deoxyATP required Acetoanaerobium sticklandii
FAD stimulates Acetoanaerobium sticklandii