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Literature summary for 5.4.3.2 extracted from

  • Hung, C.C.; Lai, M.C.
    The phylogenetic analysis and putative function of lysine 2,3-aminomutase from methanoarchaea infers the potential biocatalysts for the synthesis of beta-lysine (2013), J. Microbiol. Immunol. Infect., 46, 1-10.
    View publication on PubMed
Search for more literature for 5.4.3.2

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine functions as a hydrogen transfer agent in this aminomutase reaction Methanosarcina mazei
S-adenosyl-L-methionine functions as a hydrogen transfer agent in this aminomutase reaction Methanocalculus chunghsingensis
S-adenosyl-L-methionine functions as a hydrogen transfer agent in this aminomutase reaction Methanohalophilus portucalensis

Application

Application Comment Organism
synthesis lysine 2,3-aminomutase from methanoarchaea can act as potential in vivo and in vitro biocatalyst for the synthesis of beta-lysine and related pharmacologically active compounds Methanosarcina mazei
synthesis lysine 2,3-aminomutase from methanoarchaea can act as potential in vivo and in vitro biocatalyst for the synthesis of beta-lysine and related pharmacologically active compounds Methanocalculus chunghsingensis
synthesis lysine 2,3-aminomutase from methanoarchaea can act as potential in vivo and in vitro biocatalyst for the synthesis of beta-lysine and related pharmacologically active compounds Methanohalophilus portucalensis

Cloned(Commentary)

Cloned (Comment) Organism
gene ablA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) Methanosarcina mazei
gene ablA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) Methanocalculus chunghsingensis
gene ablA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) Methanohalophilus portucalensis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains an iron-sulfur cluster Methanosarcina mazei
Fe2+ the enzyme contains an iron-sulfur cluster Methanocalculus chunghsingensis
Fe2+ the enzyme contains an iron-sulfur cluster Methanohalophilus portucalensis
Zn2+ activates, the enzyme contains a zinc binding site Methanosarcina mazei
Zn2+ activates, the enzyme contains a zinc binding site Methanocalculus chunghsingensis
Zn2+ activates, the enzyme contains a zinc binding site Methanohalophilus portucalensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine Methanosarcina mazei
-
 (3S)-3,6-diaminohexanoate
-
 r
L-lysine Methanocalculus chunghsingensis
-
 (3S)-3,6-diaminohexanoate
-
 r
L-lysine Methanohalophilus portucalensis conversion of alpha-lysine to beta-lysine is confirmed by NMR analysis in strain FDF1 (3S)-3,6-diaminohexanoate
-
 r
L-lysine Methanosarcina mazei DSM 3647
-
 (3S)-3,6-diaminohexanoate
-
 r
L-lysine Methanohalophilus portucalensis FDF1 conversion of alpha-lysine to beta-lysine is confirmed by NMR analysis in strain FDF1 (3S)-3,6-diaminohexanoate
-
 r
L-lysine Methanocalculus chunghsingensis K1F9705b
-
 (3S)-3,6-diaminohexanoate
-
 r
additional information Methanosarcina mazei lysine 2,3-aminomutases functions as potential biocatalysts for the synthesis of beta-lysine in vivo and in vitro ?
-
 ?
additional information Methanosarcina mazei DSM 3647 lysine 2,3-aminomutases functions as potential biocatalysts for the synthesis of beta-lysine in vivo and in vitro ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocalculus chunghsingensis G3F9W8 gene ablA
-
Methanocalculus chunghsingensis K1F9705b G3F9W8 gene ablA
-
Methanohalophilus portucalensis G3F9X2 gene ablA
-
Methanohalophilus portucalensis FDF1 G3F9X2 gene ablA
-
Methanosarcina mazei Q8PYC9 gene ablA
-
Methanosarcina mazei DSM 3647 Q8PYC9 gene ablA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine
-
 Methanosarcina mazei (3S)-3,6-diaminohexanoate
-
 r
L-lysine
-
 Methanocalculus chunghsingensis (3S)-3,6-diaminohexanoate
-
 r
L-lysine conversion of alpha-lysine to beta-lysine is confirmed by NMR analysis in strain FDF1 Methanohalophilus portucalensis (3S)-3,6-diaminohexanoate
-
 r
L-lysine the enzyme functions by radical mechanism Methanosarcina mazei (3S)-3,6-diaminohexanoate
-
 r
L-lysine the enzyme functions by radical mechanism Methanocalculus chunghsingensis (3S)-3,6-diaminohexanoate
-
 r
L-lysine the enzyme functions by radical mechanism Methanohalophilus portucalensis (3S)-3,6-diaminohexanoate
-
 r
L-lysine
-
 Methanosarcina mazei DSM 3647 (3S)-3,6-diaminohexanoate
-
 r
L-lysine the enzyme functions by radical mechanism Methanosarcina mazei DSM 3647 (3S)-3,6-diaminohexanoate
-
 r
L-lysine conversion of alpha-lysine to beta-lysine is confirmed by NMR analysis in strain FDF1 Methanohalophilus portucalensis FDF1 (3S)-3,6-diaminohexanoate
-
 r
L-lysine the enzyme functions by radical mechanism Methanohalophilus portucalensis FDF1 (3S)-3,6-diaminohexanoate
-
 r
L-lysine
-
 Methanocalculus chunghsingensis K1F9705b (3S)-3,6-diaminohexanoate
-
 r
L-lysine the enzyme functions by radical mechanism Methanocalculus chunghsingensis K1F9705b (3S)-3,6-diaminohexanoate
-
 r
additional information lysine 2,3-aminomutases functions as potential biocatalysts for the synthesis of beta-lysine in vivo and in vitro Methanosarcina mazei ?
-
 ?
additional information lysine 2,3-aminomutases functions as potential biocatalysts for the synthesis of beta-lysine in vivo and in vitro Methanosarcina mazei DSM 3647 ?
-
 ?

Subunits

Subunits Comment Organism
More three-dimensional structure anaysis, docking study and modeling, structure comparisons, overview Methanosarcina mazei
More three-dimensional structure anaysis, docking study and modeling, structure comparisons, overview Methanocalculus chunghsingensis
More three-dimensional structure anaysis, docking study and modeling, structure comparisons, overview Methanohalophilus portucalensis

Synonyms

Synonyms Comment Organism
AblA
-
 Methanosarcina mazei
AblA
-
 Methanocalculus chunghsingensis
AblA
-
 Methanohalophilus portucalensis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate the cofactor facilitates the rearrangement of a substrate radical Methanosarcina mazei
pyridoxal 5'-phosphate the cofactor facilitates the rearrangement of a substrate radical Methanocalculus chunghsingensis
pyridoxal 5'-phosphate the cofactor facilitates the rearrangement of a substrate radical Methanohalophilus portucalensis

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of beta-lysine, in methanoarchaea, beta-lysine acts as a precursor for osmolyte Nepsilon-acetyl-beta-lysine in response to abiotic salt and osmotic stress Methanosarcina mazei
physiological function the enzyme is involved in biosynthesis of beta-lysine, in methanoarchaea, beta-lysine acts as a precursor for osmolyte Nepsilon-acetyl-beta-lysine in response to abiotic salt and osmotic stress Methanocalculus chunghsingensis
physiological function the enzyme is involved in biosynthesis of beta-lysine, in methanoarchaea, beta-lysine acts as a precursor for osmolyte Nepsilon-acetyl-beta-lysine in response to abiotic salt and osmotic stress Methanohalophilus portucalensis