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Literature summary for 5.4.3.11 extracted from

  • Kudo, F.; Kawamura, K.; Uchino, A.; Miyanaga, A.; Numakura, M.; Takayanagi, R.; Eguchi, T.
    Genome mining of the hitachimycin biosynthetic gene cluster involvement of a phenylalanine-2,3-aminomutase in biosynthesis (2015), ChemBioChem, 16, 909-914 .
    View publication on PubMed
Search for more literature for 5.4.3.11

Cloned(Commentary)

Cloned (Comment) Organism
gene hitA, cDNA library construction, genome mining of hitachimycin biosynthetic gene cluster, DNA and amino acid sequence determination and analysis. The identified gene cluster is responsible for the biosynthesis of hitachimycin. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Embleya scabrispora

Protein Variants

Protein Variants Comment Organism
additional information construction of a hitA knockout mutant strain Embleya scabrispora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine Embleya scabrispora
-
 D-beta-phenylalanine
-
 ?
L-phenylalanine Embleya scabrispora JCM 11712 / DSM 41855
-
 D-beta-phenylalanine
-
 ?

Organism

Organism UniProt Comment Textmining
Embleya scabrispora A0A0F7R1D9
-
-
Embleya scabrispora JCM 11712 / DSM 41855 A0A0F7R1D9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration Embleya scabrispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
 Embleya scabrispora D-beta-phenylalanine
-
 ?
L-phenylalanine
-
 Embleya scabrispora JCM 11712 / DSM 41855 D-beta-phenylalanine
-
 ?

Synonyms

Synonyms Comment Organism
HitA
-
 Embleya scabrispora
PAM
-
 Embleya scabrispora
phenylalanine-2,3-aminomutase
-
 Embleya scabrispora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Embleya scabrispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Embleya scabrispora

General Information

General Information Comment Organism
malfunction a hitA knockout mutant shows no hitachimycin production, but antibiotic production is restored by feeding with (S)-beta-Phe Embleya scabrispora
metabolism the enzyme is important in the hitachimycin biosynthetic pathway. A plausible biosynthetic pathway for hitachimycin, including a unique polyketide skeletal transformation mechanism, is proposed Embleya scabrispora