Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pantoea agglomerans |
expression in Escherichia coli | Streptomyces maritimus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
L-phenylalanine | 25°C, pH not specified in the publication | Streptomyces maritimus | |
0.027 | - |
D-beta-phenylalanine | 25°C, pH not specified in the publication | Pantoea agglomerans | |
0.032 | - |
L-phenylalanine | 25°C, pH not specified in the publication | Pantoea agglomerans | |
0.04 | - |
D-beta-phenylalanine | 25°C, pH not specified in the publication | Streptomyces maritimus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | Q84FL5 | - |
- |
Streptomyces maritimus | Q9KHJ9 | putative | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-beta-phenylalanine | - |
Pantoea agglomerans | L-phenylalanine | - |
r | |
D-beta-phenylalanine | - |
Streptomyces maritimus | L-phenylalanine | - |
r | |
L-phenylalanine | - |
Pantoea agglomerans | D-beta-phenylalanine | - |
r | |
L-phenylalanine | - |
Streptomyces maritimus | D-beta-phenylalanine | - |
r | |
additional information | enzyme additionally displays ammonia lyase activity, EC. 4.3.1.24, catalyzing the elimination of ammonia from L-phenylalanine to give trans-cinnamate. No trans-cinnamate is produced upon incubation with L-phenylalanine at 25°C, whilst very low lyase activity can be observed at 30°C | Pantoea agglomerans | ? | - |
? | |
additional information | enzyme additionally displays ammonia lyase activity, EC. 4.3.1.24, catalyzing the elimination of ammonia from L-phenylalanine to give trans-cinnamate. No trans-cinnamate is produced upon incubation with L-phenylalanine at 25°C, whilst very low lyase activity can be observed at 30°C | Streptomyces maritimus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EncP | - |
Streptomyces maritimus |
PAM | - |
Pantoea agglomerans |
PAM | - |
Streptomyces maritimus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the ammonia lyase activity shows a temperature maximum at 70°C | Pantoea agglomerans |
additional information | - |
the ammonia lyase activity shows a temperature maximum at about 75°C | Streptomyces maritimus |
45 | - |
aminomutase activity | Streptomyces maritimus |
50 | - |
aminomutase activity | Pantoea agglomerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
3 h, no loss of activity | Streptomyces maritimus |
65 | - |
3 h, 60% residual activity | Streptomyces maritimus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
D-beta-phenylalanine | 25°C, pH not specified in the publication | Pantoea agglomerans | |
0.087 | - |
L-phenylalanine | 25°C, pH not specified in the publication | Pantoea agglomerans | |
0.159 | - |
L-phenylalanine | 25°C, pH not specified in the publication | Streptomyces maritimus | |
0.22 | - |
D-beta-phenylalanine | 25°C, pH not specified in the publication | Streptomyces maritimus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
D-beta-phenylalanine | 25°C, pH not specified in the publication | Pantoea agglomerans | |
2.73 | - |
L-phenylalanine | 25°C, pH not specified in the publication | Pantoea agglomerans | |
5.53 | - |
D-beta-phenylalanine | 25°C, pH not specified in the publication | Streptomyces maritimus | |
7.19 | - |
L-phenylalanine | 25°C, pH not specified in the publication | Streptomyces maritimus |