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Literature summary for 5.4.2.9 extracted from

  • Seidel, H.M.; Freeman, S.; Seto, H.; Knowles, J.R.
    Phosphonate biosynthesis: isolation of the enzyme responsible for the formation of a carbon-phosphorus bond (1988), Nature, 335, 457-458.
    View publication on PubMed
Search for more literature for 5.4.2.9

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Tetrahymena pyriformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
 Phosphonopyruvate 30°C, pH 7.7 Tetrahymena pyriformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 2 * 33000, denaturing gel electrophoresis Tetrahymena pyriformis
69000
-
 non-denaturing gel electrophoresis Tetrahymena pyriformis

Organism

Organism UniProt Comment Textmining
Tetrahymena pyriformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tetrahymena pyriformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Tetrahymena pyriformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate equilibrium greatly favours phosphoenolpyruvate Tetrahymena pyriformis 3-phosphonopyruvate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 33000, denaturing gel electrophoresis Tetrahymena pyriformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Tetrahymena pyriformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41
-
 Phosphonopyruvate 30°C, pH 7.7 Tetrahymena pyriformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Tetrahymena pyriformis