Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | similar enzyme | Streptomyces hygroscopicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | increases activity | Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE, similar enzyme | Streptomyces hygroscopicus |
45000 | 67000 | Streptomyces hygroscopicus SF1293, gel filtration, similar enzyme | Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate | Streptomyces hygroscopicus | enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 | 3-phosphonopyruvate | - |
? | |
phosphoenolpyruvate | Streptomyces hygroscopicus SF1293 | enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 | 3-phosphonopyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | - |
similar enzyme | - |
Streptomyces hygroscopicus | - |
SF1293 | - |
Streptomyces hygroscopicus | - |
mutant NP71 | - |
Streptomyces hygroscopicus SF1293 | - |
SF1293 | - |
Purification (Comment) | Organism |
---|---|
SF1293, mutant NP71 | Streptomyces hygroscopicus |
similar enzyme | Streptomyces hygroscopicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces hygroscopicus |
Storage Stability | Organism |
---|---|
-20°C, 80% ammonium sulfate suspension, stable for 1 month, similar enzyme | Streptomyces hygroscopicus |
4°C, 50 mM MES buffer, pH 6.5, 1 mg protein/ml, stable for 1 week, similar enzyme | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus | phosphinopyruvate + CO2 | - |
? | |
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus SF1293 | phosphinopyruvate + CO2 | - |
? | |
phosphoenolpyruvate | enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 | Streptomyces hygroscopicus | 3-phosphonopyruvate | - |
? | |
phosphoenolpyruvate | enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 | Streptomyces hygroscopicus SF1293 | 3-phosphonopyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE, similar enzyme | Streptomyces hygroscopicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
similar enzyme | Streptomyces hygroscopicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
similar enzyme | Streptomyces hygroscopicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | stable in this range, similar enzyme | Streptomyces hygroscopicus |