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Literature summary for 5.4.2.8 extracted from

  • Kaur, T.; Ghosh, M.
    Characterization and upregulation of bifunctional phosphoglucomutase/phosphomannomutase enzyme in an exobiopolymer overproducing strain of Acinetobacter haemolyticus (2015), Microbiol. Res., 181, 8-14 .
    View publication on PubMed
Search for more literature for 5.4.2.8

Inhibitors

Inhibitors Comment Organism Structure
ADP 50% inhibition at 5 mM Acinetobacter haemolyticus
ATP 20% inhibition at 5 mM Acinetobacter haemolyticus
Ca2+ 50% inhibition at 5 mM Acinetobacter haemolyticus
Co2+ 78% inhibition at 5 mM Acinetobacter haemolyticus
D-fructose 6-phosphate 38% inhibition at 5 mM Acinetobacter haemolyticus
D-Glucose 1,6-bisphosphate 90% inhibition at 5 mM Acinetobacter haemolyticus
Li+ complete inhibition at 5 mM Acinetobacter haemolyticus
additional information not inhibited by Mg2+, D-glucose 6-phosphate, and EDTA Acinetobacter haemolyticus
Ni2+ 96% inhibition at 5 mM Acinetobacter haemolyticus
UDP-D-glucose 65% inhibition at 5 mM Acinetobacter haemolyticus
Zn2+ complete inhibition at 5 mM Acinetobacter haemolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 alpha-D-mannose 1-phosphate at pH 7.5 and 35°C Acinetobacter haemolyticus
0.028
-
 alpha-D-glucose 1-phosphate at pH 7.5 and 35°C Acinetobacter haemolyticus

Organism

Organism UniProt Comment Textmining
Acinetobacter haemolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, ion exchange column chromatography, and Sephacryl S-100 gel filtration Acinetobacter haemolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45.1
-
 cell lysate, at pH 7.5 and 35°C Acinetobacter haemolyticus
1548
-
 after 34.4fold purification, at pH 7.5 and 35°C Acinetobacter haemolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
 Acinetobacter haemolyticus D-glucose 6-phosphate
-
 ?
alpha-D-mannose 1-phosphate
-
 Acinetobacter haemolyticus D-mannose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Acinetobacter haemolyticus

Synonyms

Synonyms Comment Organism
PGM/PMM bifunctional enzyme Acinetobacter haemolyticus
phosphoglucomutase/phosphomannomutase bifunctional enzyme Acinetobacter haemolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Acinetobacter haemolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 about 10% activity at 10°C, about 30% activity at 20°C, about 60% activity at 25°C, about 80% activity at 30°C, 100% activity at 35°C, about 35% activity at 40°C, about 10% activity at 50°C Acinetobacter haemolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Acinetobacter haemolyticus

pH Range

pH Minimum pH Maximum Comment Organism
3 9 about 3% activity at pH 3.0-4.0, about 10% activity at pH 5.0, about 80% activity at pH 6.0, 100% activity at pH 7.5, about 30% activity at pH 8.0, about 10% activity at pH 9.0 Acinetobacter haemolyticus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5
-
 at pH 7.5 and 35°C Acinetobacter haemolyticus ADP
5
-
 at pH 7.5 and 35°C Acinetobacter haemolyticus Ca2+