Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-Glucose 1,6-bisphosphate | in presence of 0.02 and 0.2 mM mannose 1-phosphate substrate, half-maximal stimulation of recombinant enzyme at 0.004 and 0.008 mM D-glucose 1,6-bisphosphate | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
- |
Nicotiana benthamiana |
expression in Escherichia coli with His-tag, expression as green fluorescent protein fusion protein in Arabidospsis thaliana | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression as green fluorescent protein fusion protein in Arabidospsis thaliana increases ascorbic acid content by 25-33% | Arabidopsis thaliana |
additional information | reducing enzyme expression level through virus-induced gene silencing causes substantial decrease in ascorbic acid content in leaves. Raising the expression level leads to 20-50% increase in ascorbic acid content | Nicotiana benthamiana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0297 | - |
D-mannose 1-phosphate | 30°C, pH 7.5 | Arabidopsis thaliana | |
0.0654 | - |
D-glucose 1-phosphate | 30°C, pH 7.5 | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27700 | - |
x * 27700, calculated and MALDI-TOF | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O80840 | - |
- |
Nicotiana benthamiana | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme is constitutively expressed in both vegetative and reproductive organs | Nicotiana benthamiana | - |
additional information | enzyme is constitutively expressed in both vegetative and reproductive organs | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 1-phosphate | - |
Nicotiana benthamiana | D-glucose 6-phosphate | - |
? | |
D-glucose 1-phosphate | reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate | Arabidopsis thaliana | D-glucose 6-phosphate | - |
? | |
D-Mannose 1-phosphate | - |
Nicotiana benthamiana | D-Mannose 6-phosphate | - |
r | |
D-Mannose 1-phosphate | reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate | Arabidopsis thaliana | D-Mannose 6-phosphate | - |
r | |
D-mannose 6-phosphate | - |
Nicotiana benthamiana | D-mannose 1-phosphate | - |
r | |
D-mannose 6-phosphate | - |
Arabidopsis thaliana | D-mannose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 27700, calculated and MALDI-TOF | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
95% of maximum activity | Arabidopsis thaliana |
7.5 | - |
- |
Arabidopsis thaliana |