Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-D-glucose 1,6-diphosphate | required | Amorphophallus konjac |
General Stability | Organism |
---|---|
Mg2+ stabilizes | Amorphophallus konjac |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Be2+ | in histidine buffer | Amorphophallus konjac | |
Co2+ | weak | Amorphophallus konjac | |
Cu2+ | in Tris buffer, no inhibition in histidine buffer | Amorphophallus konjac | |
mannose 1-phosphate | at high concentrations, competitive with respect to glucose-1,6-diphosphate, Ki: 1.2 mM | Amorphophallus konjac | |
Ni2+ | weak | Amorphophallus konjac | |
Zn2+ | in Tris buffer, no inhibition in histidine buffer | Amorphophallus konjac |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
mannose 1-phosphate | - |
Amorphophallus konjac |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 72% of the activation relative to Mg2+, maximal activity at 0.5 mM | Amorphophallus konjac | |
Mg2+ | required | Amorphophallus konjac | |
Mg2+ | maximal activity at 1 mM | Amorphophallus konjac | |
Ni2+ | 36% of the activation relative to Mg2+, maximal activity at 0.5 mM | Amorphophallus konjac |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | Amorphophallus konjac |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amorphophallus konjac | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Amorphophallus konjac |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
corm | - |
Amorphophallus konjac | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.105 | - |
- |
Amorphophallus konjac |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 2 months | Amorphophallus konjac |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 1-phosphate | - |
Amorphophallus konjac | D-Mannose 6-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Amorphophallus konjac |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
50% loss of activity within a few min, completely protected from heat inactivation by 1 mM mannose 1-phosphate, 0.01 mM glucose 1,6-diphosphate or 1 mM fructose 1,6-diphosphate. In presence of 1 mM Mg2+: 50% loss of activity after 17 min | Amorphophallus konjac |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Amorphophallus konjac |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
maximal stability | Amorphophallus konjac |