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Literature summary for 5.4.2.7 extracted from
- Biosynthesis of an antiviral compound using a stabilized phosphopentomutase by multipoint covalent immobilization (2017), J. Biotechnol., 249, 34-41 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-Glucose 1,6-bisphosphate | about 7fold increase of activity at 0.001 mM | Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| using 10% (v/v) glycerol, the enzyme maintains 86% of its initial activity at pH 10.0 after 18 h incubation | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | highest activity at 0.1 mM | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-ribose 1-phosphate | Escherichia coli | - |
D-ribose 5-phosphate | - |
? | |
| alpha-D-ribose 1-phosphate | Escherichia coli ATCC 4157 | - |
D-ribose 5-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli ATCC 4157 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-ribose 1-phosphate | - |
Escherichia coli | D-ribose 5-phosphate | - |
? | |
| alpha-D-ribose 1-phosphate | - |
Escherichia coli ATCC 4157 | D-ribose 5-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPM | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme immobilized on CNBr-Ag is completely inactive after 16 h incubation at 50°C. Enzymes immobilized on cyanogen bromide or glyoxyl agarose maintain 100% of their initial activity after 16 h at 50°C. Enzyme immobilized on monoaminoethyl-N-aminoethyl-glutaraldehyde or monoaminoethyl-N-aminoethyl-agarose loses more than 50% of its initial activity after 16 h at 50°C | Escherichia coli |
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