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Literature summary for 5.4.2.7 extracted from

  • Moustafa, H.M.; Zaghloul, T.I.; Zhang, Y.H.
    A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima (2016), Anal. Biochem., 501, 75-81 .
    View publication on PubMed
Search for more literature for 5.4.2.7

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 100% activity at 0.5 mM Thermotoga maritima
alpha-D-glucose1,6-bisphosphate
-
 Thermotoga maritima
dithiothreitol 100% activity at 0.5 mM Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 alpha-D-ribose 5-phosphate at pH 7.5 and 60°C Thermotoga maritima
1.2
-
 alpha-D-ribose 5-phosphate at pH 7.5 and 90°C Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required, 100% activity at 0.1 mM Thermotoga maritima
additional information the presence of Ni2+, Co2+, Zn2+, and Cu2+ enhances enzyme activities, but not as much as Mn2+ Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-ribose 1-phosphate Thermotoga maritima
-
 alpha-D-ribose 5-phosphate
-
 r
alpha-D-ribose 5-phosphate Thermotoga maritima
-
 alpha-D-ribose 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WY14
-
-

Purification (Commentary)

Purification (Comment) Organism
IMAC Ni-charged resin column chromatography, and Superdex 200 gel filtration Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
 crude enzyme, at pH 7.5 and 90°C Thermotoga maritima
57.8
-
 after 4.1fold purification, at pH 7.5 and 90°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-ribose 1-phosphate
-
 Thermotoga maritima alpha-D-ribose 5-phosphate
-
 r
alpha-D-ribose 5-phosphate
-
 Thermotoga maritima alpha-D-ribose 1-phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
alpha-D-ribose1,5-phosphomutase
-
 Thermotoga maritima
PPM
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100 about 30% activity at 30°C, about 40% activity at 40°C, about 50% activity at 50°C, about 60% activity at 60°C, about 70% activity at 70°C, about 80% activity at 80°C, 100% activity at 90°C, about 90% activity at 100°C Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
55.6
-
 alpha-D-ribose 5-phosphate at pH 7.5 and 60°C Thermotoga maritima
185
-
 alpha-D-ribose 5-phosphate at pH 7.5 and 90°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in 100 mM MOPS buffer Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 about 40% activity at pH 6.5, more than 70% activity between pH 7.0 and 8.0 Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
74.1
-
 alpha-D-ribose 5-phosphate at pH 7.5 and 60°C Thermotoga maritima
154
-
 alpha-D-ribose 5-phosphate at pH 7.5 and 90°C Thermotoga maritima