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Literature summary for 5.4.2.7 extracted from
- Purification and characterization of phosphopentomutase from rat liver (1983), Biochim. Biophys. Acta, 743, 162-171.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cys | activates | Rattus norvegicus |  |
| glucose 1,6-diphosphate | activates, optimal concentration 0.01 mM | Rattus norvegicus | |
General Stability
| General Stability | Organism |
|---|---|
| glucose 1,6-diphosphate protects against thermal inactivation | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AsO43- | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
deoxyribose 1-phosphate | - |
Rattus norvegicus | |
| 0.13 | - |
ribose 1-phosphate | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates | Rattus norvegicus | |
| Mg2+ | optimal concentration 0.2 mM | Rattus norvegicus | |
| Mn2+ | stimulates, optimal concentration is 0.2 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32500 | - |
2 * 32500, SDS-PAGE | Rattus norvegicus |
| 65000 | - |
disc gel electrophoresis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, Tris buffer or phosphate buffer, pH 7-8, stable for 1 month | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribose 1-phosphate | - |
Rattus norvegicus | D-ribose 5-phosphate | - |
? | |
| Deoxyribose 1-phosphate | at 25% of the activity relative to ribose 1-phosphate | Rattus norvegicus | Deoxyribose 5-phosphate | - |
? | |
| Glucose 1-phosphate | at 2.4% of the activity relative to ribose 1-phosphate | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32500, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 8, rapid loss of activity in absence of glucose 1,6-diphosphate, 70% loss of activity after 10 min, complete loss of activity after 1 h. Completely stable for 90 min in presence of 11 mM glucose 1,6-diphosphate | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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