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Literature summary for 5.4.2.7 extracted from
- Phosphodeoxyribomutase from Escherichia coli. Purification and some properties (1970), Eur. J. Biochem., 17, 397-407.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Deoxyribose-1,5-diphosphate | 10fold stimulation, Km: 0.00017 mM, optimal concentration: 0.0014 mM | Escherichia coli |  |
| glucose 1,6-diphosphate | 3fold stimulation, Km: 0.00008 mM, optimal concentration: 0.0007 mM | Escherichia coli | |
| ribose-1,5-diphosphate | 10fold stimulation, Km: 0.00017 mM, optimal concentration: 0.0014 mM | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | in presence of 0.2 mM CoCl2 | Escherichia coli | |
| Cd2+ | in presence of 0.2 mM CoCl2 | Escherichia coli | |
| Cu2+ | in presence of 0.2 mM CoCl2 | Escherichia coli | |
| Fe3+ | in presence of 0.2 mM CoCl2 | Escherichia coli | |
| phosphate | 50 mM, 95% inhibition | Escherichia coli | |
| SO42- | 50 mM, 85% inhibition | Escherichia coli | |
| Zn2+ | in presence of 0.2 mM CoCl2 | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
deoxyribose 1-phosphate | - |
Escherichia coli | |
| 0.043 | - |
ribose 1-phosphate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activity is dependent on Mn2+, Ni2+ or Co2+. Optimal concentration: 0.05-0.06 mM | Escherichia coli | |
| Mn2+ | activity is dependent on Mn2+, Ni2+ or Co2+. Optimal concentration: 0.05-0.06 mM | Escherichia coli | |
| Ni2+ | activity is dependent on Mn2+, Ni2+ or Co2+. Optimal concentration: 0.05 mM with deoxyribose 1-phosphate and 0.09 mM with ribose 1-phosphate as substrate | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration | Escherichia coli |
| 43000 | - |
1 * 43000, enzyme form I, II and III, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12, mutant dra-thy- | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.5 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribose 1-phosphate | - |
Escherichia coli | D-ribose 5-phosphate | - |
? | |
| Deoxyribose 1-phosphate | - |
Escherichia coli | Deoxyribose 5-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, enzyme form I, II and III, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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