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Literature summary for 5.4.2.6 extracted from

  • Lahiri, S.D.; Zhang, G.; Dunaway-Mariano, D.; Allen, K.N.
    Caught in the act: the structure of phosphorylated beta-phosphoglucomutase from Lactococcus lactis (2002), Biochemistry, 41, 8351-8359.
    View publication on PubMed
Search for more literature for 5.4.2.6

Activating Compound

Activating Compound Comment Organism Structure
D-glucose-1,6-bisphosphate
-
 Lactococcus lactis

Cloned(Commentary)

Cloned (Comment) Organism
expression of seleno-methionine beta-PGM in Escherichia coli Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of phosphorylated native beta-PGM are grown at pH 7.5 in the presence of 5 M Mg2+ using the vapor-diffusion method with hanging drop geometry, 100 mM ammonium fluoride and 16% polyethylene glycol 3350 are used as precipitating agents, crystals diffrakt to 2.3 A Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-Glucose 1-phosphate Lactococcus lactis
-
 beta-D-Glucose 6-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Lactococcus lactis P71447
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein catalysis of reaction proceeds via a phosphoenzyme form Lactococcus lactis

Purification (Commentary)

Purification (Comment) Organism
recombinant seleno-methionine beta-PGM Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-Glucose 1-phosphate
-
 Lactococcus lactis beta-D-Glucose 6-phosphate
-
 r

Synonyms

Synonyms Comment Organism
beta-PGM
-
 Lactococcus lactis