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Literature summary for 5.4.2.6 extracted from

  • Belocopitow, E.; Marechal, L.R.
    Metabolism of trehalose in Euglena gracilis. Partial purification and some properties of phosphoglucomutase acting on beta-glucose 1-phosphate (1974), Eur. J. Biochem., 46, 631-637.
    View publication on PubMed
Search for more literature for 5.4.2.6

Activating Compound

Activating Compound Comment Organism Structure
arsenate increasemM s activity Euglena gracilis
beta-D-Glucose 1,6-diphosphate required Euglena gracilis
beta-D-Glucose 1,6-diphosphate Km: 0.0005 mM Euglena gracilis

General Stability

General Stability Organism
after 12 h in 0.02 M imidazole buffer, at pH 7.0, totally inactivated both at 0°C and at -20°C. Glycylglycine, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid and phosphate at the same conditions are able to preserve most of the enzyme activity. Cacodylate, ethylmorpholidate, Tris-maleate and glycerophosphate have an intermediate action Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
KF 10 mM: 75% inhibition, 17 mM: 100% inhibition Euglena gracilis
phosphate 10 mM, 20% inhibition Euglena gracilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 0.4 mM Co2+ Euglena gracilis
Mg2+ divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 1-3 mM Mg2+ Euglena gracilis
Mn2+ divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 0.4 mM Mn2+ Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-Glucose 1-phosphate Euglena gracilis the enzyme together with a trehalose phosphorylase would constitute a new catabolic pathway for trehalose ?
-
 ?

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.463
-
-
 Euglena gracilis

Storage Stability

Storage Stability Organism
-70°C, 0.02 M phosphate buffer, pH 7.0, stable for at least 6 months Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-Glucose 1-phosphate r Euglena gracilis beta-D-Glucose 6-phosphate
-
 ?
beta-D-Glucose 1-phosphate the enzyme together with a trehalose phosphorylase would constitute a new catabolic pathway for trehalose Euglena gracilis ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Euglena gracilis

pH Range

pH Minimum pH Maximum Comment Organism
6 8.2 6.0: about 35% of maximal activity, pH 8.2: about 15% of maximal activity Euglena gracilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 0°C: about 70% loss of activity after 2 days, -20°C: about 30% loss of activity after 2 days, -70°C: no loss of activity after 2 days Euglena gracilis