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Literature summary for 5.4.2.5 extracted from
- D-Glucose-1-phosphate:D-glucose-6-phosphotransferase (1965), Biochim. Biophys. Acta, 96, 91-101.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect on the rate of reaction found for cysteine, glutathione, 2-mercaptoethanol, glucose 1,6-diphosphate, ribose 1,5-diphosphate | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | strong inhibitor | Mycolicibacterium smegmatis |  |
| iodoacetate | - |
Mycolicibacterium smegmatis | |
| additional information | no inhibition by NaN3 | Mycolicibacterium smegmatis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
alpha-D-glucose 1-phosphate | direct assay with unreacted glucose 1-phosphate | Escherichia coli | |
| 1.1 | - |
alpha-D-glucose 1-phosphate | assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation | Escherichia coli | |
| 8.5 | - |
alpha-D-glucose | assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation | Escherichia coli | |
| 17 | - |
alpha-D-glucose | direct assay with unreacted glucose 1-phosphate | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for Mg2+, Mn2+, Fe2+ | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-Glucose 1-phosphate + D-glucose | Escherichia coli | - |
? | - |
? | |
| alpha-D-Glucose 1-phosphate + D-glucose | Mycolicibacterium smegmatis | - |
? | - |
? | |
| alpha-D-Glucose 1-phosphate + D-glucose | Klebsiella aerogenes | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Klebsiella aerogenes | - |
- |
- |
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 114 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 3 years stable | Mycolicibacterium smegmatis |
| 0°C, 3 months stable | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Deoxy-D-glucose + alpha-D-glucose 1-phosphate | - |
Escherichia coli | D-Glucose + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| 3-O-Methyl-D-glucose + alpha-D-glucose 1-phosphate | - |
Escherichia coli | 3-O-Methyl-D-glucose 6-phosphate + D-glucose | - |
? | |
| alpha-D-glucose 1-phosphate + alpha-D-glucose | - |
Escherichia coli | ? | - |
? | |
| alpha-D-Glucose 1-phosphate + D-glucose | - |
Escherichia coli | D-Glucose + alpha-D-glucose 6-phosphate | - |
? | |
| alpha-D-Glucose 1-phosphate + D-glucose | - |
Mycolicibacterium smegmatis | D-Glucose + alpha-D-glucose 6-phosphate | - |
? | |
| alpha-D-Glucose 1-phosphate + D-glucose | - |
Klebsiella aerogenes | D-Glucose + alpha-D-glucose 6-phosphate | - |
? | |
| alpha-D-Glucose 1-phosphate + D-glucose | - |
Escherichia coli | ? | - |
? | |
| alpha-D-Glucose 1-phosphate + D-glucose | - |
Mycolicibacterium smegmatis | ? | - |
? | |
| alpha-D-Glucose 1-phosphate + D-glucose | - |
Klebsiella aerogenes | ? | - |
? | |
| alpha-Methyl-D-glucoside + alpha-D-glucose 1-phosphate | - |
Escherichia coli | alpha-Methyl-D-glucoside 6-phosphate + D-glucose | - |
? | |
| beta-Methyl-D-glucoside + alpha-D-glucose 1-phosphate | - |
Escherichia coli | beta-Methyl-D-glucoside 6-phosphate + D-glucose | - |
? | |
| D-Fructose + alpha-D-glucose 1-phosphate | - |
Escherichia coli | D-Fructose 6-phosphate + D-glucose | - |
? | |
| D-Galactose + alpha-D-glucose 1-phosphate | - |
Escherichia coli | D-Galactose 6-phosphate + D-glucose | - |
? | |
| D-Mannose + alpha-D-glucose 1-phosphate | - |
Escherichia coli | D-Mannose 6-phosphate + D-glucose | - |
? | |
| Fructose 1-phosphate + D-glucose | - |
Escherichia coli | D-glucose 6-phosphate + fructose | - |
? | |
| L-Sorbose + alpha-D-glucose 1-phosphate | - |
Escherichia coli | L-Sorbose 6-phosphate + D-glucose | - |
? | |
| additional information | no substrates are 6-deoxy-D-glucose, D-ribose, D-xylose, DL-serine, cytidine, adenosine, uridine, guanosine | Escherichia coli | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
direct assay with unreacted glucose 1-phosphate | Mycolicibacterium smegmatis |
| 6.5 | - |
assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation | Mycolicibacterium smegmatis |
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