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Literature summary for 5.4.2.3 extracted from
- Functional cloning and mutational analysis of the human cDNA for phosphoacetylglucosamine mutase: identification of the amino acid residues essential for the catalysis (2000), Biochim. Biophys. Acta, 1492, 369-376.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
| expression in Escherichia coli | Candida albicans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D276A | no activity | Homo sapiens |
| D276E | extremely low activity | Homo sapiens |
| D278A | no activity | Homo sapiens |
| D278E | no activity | Homo sapiens |
| D280A | extremely low activity | Homo sapiens |
| D70A | 65% of wild type activity | Homo sapiens |
| G277A | 79% of wild type activity | Homo sapiens |
| G499A | 0.5% of wild type activity | Homo sapiens |
| G58A | 20% of wild type activity | Homo sapiens |
| G72A | 3% of wild type activity | Homo sapiens |
| H65A | no activity | Homo sapiens |
| H65R | very low activity | Homo sapiens |
| K74A | no activity | Homo sapiens |
| N66A | 1.3% of wild type activity | Homo sapiens |
| P67A | 100% of wild type activity | Homo sapiens |
| R281A | no activity | Homo sapiens |
| R281K | no activity | Homo sapiens |
| R496A | extremely low activity | Homo sapiens |
| R498A | 8% of wild type activity | Homo sapiens |
| S64A | no activity | Homo sapiens |
| S64T | no activity | Homo sapiens |
| T500A | extremely low activity | Homo sapiens |
| T62A | 106% of wild type activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
N-acetyl-alpha-D-glucosamine | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-alpha-D-glucosamine 1-phosphate | Homo sapiens | - |
N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| N-acetyl-alpha-D-glucosamine 1-phosphate | Candida albicans | - |
N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | Q9P4V2 | - |
- |
| Homo sapiens | O95394 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant CaAgm1-GST fusion protein | Candida albicans |
| recombinant HsAgm1-GST fusion protein | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-alpha-D-glucosamine | - |
Homo sapiens | ? | - |
? | |
| N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Homo sapiens | N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Candida albicans | N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CaAGM1 | - |
Candida albicans |
| HsAGM1 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.79 | - |
N-acetyl-alpha-D-glucosamine | - |
Homo sapiens | |
| 2.94 | - |
N-acetyl-alpha-D-glucosamine | - |
Homo sapiens | |
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