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Literature summary for 5.4.2.2 extracted from

  • Waugh, B.; Sen, U.; Banerjee, R.
    Crystal structure of phosphoglucomutase from Leishmania major at 3.5 A resolution (2016), Biochimie, 121, 102-111 .
    View publication on PubMed
Search for more literature for 5.4.2.2

Activating Compound

Activating Compound Comment Organism Structure
alpha-D-Glucose 1,6-bisphosphate 2fold increase of activity at 0.0001 mM Leishmania major

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.0, 10% (w/v) PEG 3350, 10 mM MgCl2 and 0.02% (w/v) NaN3 Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02135
-
 alpha-D-glucose 1-phosphate pH and temperature not specified in the publication Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate Leishmania major
-
 D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania major Q4QCF1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
 Leishmania major D-glucose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 63896, calculated from amino acid sequence Leishmania major

Synonyms

Synonyms Comment Organism
PGM
-
 Leishmania major

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
45
-
 alpha-D-glucose 1-phosphate pH and temperature not specified in the publication Leishmania major