Cloned (Comment) | Organism |
---|---|
genes pgm1, pgm2, and pgm3, cloning in Escherichia coli strain DH5alpha, expression of His-tagged enzymes in Escherichia coli strain BL21 (DE3) | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
alpha-D-glucose 1-phosphate | recombinant His-tagged Pgm2, pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.06 | - |
alpha-D-glucose 1-phosphate | recombinant His-tagged Pgm1, pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.112 | - |
alpha-D-glucose 1-phosphate | recombinant His-tagged Pgm3, pH 7.5, 30°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | Saccharomyces cerevisiae | - |
alpha-D-glucose 6-phosphate | - |
? | |
additional information | Saccharomyces cerevisiae | Pgm3 functions as the major phosphoribomutase in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Pgm1, Pgm2, and Pgm3 from Escherichia coli strain BL21 (DE3) | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | 1 | purified recombinant His-tagged Pgm3, pH 7.5, 30°C | Saccharomyces cerevisiae |
0.24 | - |
purified recombinant His-tagged Pgm1, pH 7.5, 30°C | Saccharomyces cerevisiae |
33.7 | - |
purified recombinant His-tagged Pgm2, pH 7.5, 30°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | - |
Saccharomyces cerevisiae | alpha-D-glucose 6-phosphate | - |
? | |
additional information | Pgm3 functions as the major phosphoribomutase in vivo | Saccharomyces cerevisiae | ? | - |
? | |
additional information | phosphoglucomutases Pgm1, Pgm2, and Pgm3, EC 5.4.2.2, of Saccharomyces cerevisiae show ability to interconvert ribose-1-phosphate and ribose-5-phosphate. The purified proteins, studied in vitro with regard to their kinetic properties on glucose-1-phosphate and ribose-1-phosphate, are all active on both substrates with Pgm1 exhibiting only residual activity on ribose-1-phosphate. The Pgm2 and Pgm3 proteins have almost equal kinetic properties on ribose-1-phosphate, but Pgm2 has a 2000times higher preference for glucose-1-phosphate when compared to Pgm3 | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGM | - |
Saccharomyces cerevisiae |
PGM1 | - |
Saccharomyces cerevisiae |
PGM2 | - |
Saccharomyces cerevisiae |
PGM3 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | only mutants with a deletion of PGM3, not of PGM1 or PGM2, hyperaccumulate ribose-1-phosphate | Saccharomyces cerevisiae |