Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-D-Glucose 1,6-bisphosphate | the maximum activity is obtained in the presence of more than 0.2 mM alpha-D-glucose 1,6-bisphosphate | Toxoplasma gondii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
alpha-D-glucose 1-phosphate | isozyme PGM-I, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii | |
3.53 | - |
alpha-D-glucose 1-phosphate | isozyme PGM-II, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ are the most effective divalent cations for the enzyme activity of isozyme PGM-I | Toxoplasma gondii | |
Mg2+ | Mg2+ are the most effective divalent cations for the enzyme activity of isozyme PGM-II | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | C6L2E2 | isozyme PGM-I | - |
Toxoplasma gondii | C6L2F4 | isozyme PGM-II | - |
Toxoplasma gondii Beverley | C6L2E2 | isozyme PGM-I | - |
Toxoplasma gondii Beverley | C6L2F4 | isozyme PGM-II | - |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84 | - |
isozyme PGM-II, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii |
338 | - |
isozyme PGM-I, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | - |
Toxoplasma gondii | alpha-D-glucose 6-phosphate | - |
? | |
alpha-D-glucose 1-phosphate | - |
Toxoplasma gondii Beverley | alpha-D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-phosphoglucomutase | - |
Toxoplasma gondii |
PGM-I | isozyme | Toxoplasma gondii |
PGM-II | isozyme | Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93 | - |
alpha-D-glucose 1-phosphate | isozyme PGM-II, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii | |
398 | - |
alpha-D-glucose 1-phosphate | isozyme PGM-I, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii |