Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glucose 1,6-bisphosphate | absolutely required | Lactococcus lactis |
Cloned (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overproduction of enzyme leads to increased glycolytic flux and growth rate on galactose. Growth of a conditional knock-out strain is severely impaired when enzyme activity is below the control level | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Lactococcus lactis | |
D-fructose 1,6-bisphosphate | - |
Lactococcus lactis | |
Zn2+ | - |
Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0168 | - |
alpha-D-glucose 1-phosphate | pH 7.2, 30°C | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, less than 2% of activity in absence of Mg2+ | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
3 * 28276, calculated, 3 * 28000, SDS-PAGE | Lactococcus lactis |
28276 | - |
3 * 28276, calculated, 3 * 28000, SDS-PAGE | Lactococcus lactis |
84300 | - |
gel filtration | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q00G41 | - |
- |
Purification (Comment) | Organism |
---|---|
both native and recombinant enzyme | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | enzyme is highly specific for substrate and product | Lactococcus lactis | alpha-D-glucose 6-phosphate | - |
r | |
alpha-D-glucose 6-phosphate | 23% of the activity with alpha-D-glucose 1-phosphate | Lactococcus lactis | alpha-D-glucose 1-phosphate | - |
r | |
additional information | no substrate: alpha-D-mannose 1-phosphate, beta-D-glucose 1-phosphate | Lactococcus lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 28276, calculated, 3 * 28000, SDS-PAGE | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | 7.5 | more than 50% of maximum activity within this range | Lactococcus lactis |
6.5 | - |
- |
Lactococcus lactis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
48 | - |
D-fructose 1,6-bisphosphate | pH 7.2, 30°C | Lactococcus lactis |