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Literature summary for 5.4.2.2 extracted from

  • Daugherty, J.P.; Kraemer, W.F.; Joshi, J.G.
    Purification and properties of phosphoglucomutase from Fleischmann's yeast (1975), Eur. J. Biochem., 57, 115-126.
    View publication on PubMed
Search for more literature for 5.4.2.2

Activating Compound

Activating Compound Comment Organism Structure
alpha-D-glucose 1,6-diphosphate Km: 0.00224 mM Saccharomyces cerevisiae

General Stability

General Stability Organism
stable in 5 mM acetate buffer or 10 mM citrate buffer, pH 5.5, 4 C, but in 5 mM Tris-HCl buffer pH 7.4, 80% loss of activity per day Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Be2+
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 alpha-D-glucose 1-phosphate
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal activity with 1.0-3.0 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31633
-
 2 * 31633, sedimentation equilibrium in 6 M guanidine-HCl, sedimentation equilibrium in dodecyl sulfate and 2-mercaptoethanol, SDS-PAGE Saccharomyces cerevisiae
65600
-
 gel filtration, sucrose density gradient centrifugation, sedimentation equilibrium centrifugation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
490
-
 substrate glucose 1-phosphate Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
4°C, saturated ammonium sulfate, 0.1 M Na2HPO4-HCl, pH 7.0, 0.1 mM EDTA, 1 mM Mg2+, 3 months stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
 Saccharomyces cerevisiae D-glucose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31633, sedimentation equilibrium in 6 M guanidine-HCl, sedimentation equilibrium in dodecyl sulfate and 2-mercaptoethanol, SDS-PAGE Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
41
-
 at least 5 min stable Saccharomyces cerevisiae