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Literature summary for 5.4.2.12 extracted from

  • Haferkamp, P.
    Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus (2011), PH. D. Thesis Universität Duisburg-Essen, 2011, 0000.
No PubMed abstract available
Search for more literature for 5.4.2.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 3-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
0.1
-
 3-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
0.11
-
 3-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
0.11
-
 2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
0.15
-
 2-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
0.26
-
 2-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46800
-
 x * 46000-48000, SDS-PAGE, x * 46800, calculated Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q980A0
-
-
Saccharolobus solfataricus DSM 1617 Q980A0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Saccharolobus solfataricus 3-phospho-D-glycerate
-
 ?
2-phospho-D-glycerate
-
 Saccharolobus solfataricus DSM 1617 3-phospho-D-glycerate
-
 ?
3-phospho-D-glycerate
-
 Saccharolobus solfataricus 2-phospho-D-glycerate
-
 ?
3-phospho-D-glycerate
-
 Saccharolobus solfataricus DSM 1617 2-phospho-D-glycerate
-
 ?

Subunits

Subunits Comment Organism
? x * 46000-48000, SDS-PAGE, x * 46800, calculated Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
apgM
-
 Saccharolobus solfataricus
SSO0417
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.51
-
 3-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
2.04
-
 2-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
2.18
-
 3-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
2.31
-
 2-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
3.01
-
 3-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
4.01
-
 2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.84
-
 2-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
15.4
-
 2-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
19.81
-
 3-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
27.36
-
 3-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
35.95
-
 3-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
36.45
-
 2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus