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Literature summary for 5.4.2.12 extracted from
- Identification and characterization of the cofactor-independent phosphoglycerate mutases of Dirofilaria immitis and its Wolbachia endosymbiont (2011), Vet. Parasitol., 176, 350-356.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | independent on 2,3-bisphosphoglycerate | Dirofilaria immitis | |
| additional information | independent on 2,3-bisphosphoglycerate | Wolbachia sp. |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C2566/T7 cells | Wolbachia endosymbiont of Dirofilaria immitis |
| expressed in Escherichia coli C2566/T7 cells | Dirofilaria immitis |
| expression of C-terminally His-tagged enzyme in Escherichia coli strain C2566/T7 | Wolbachia sp. |
| expression of C-terminally His6-tagged enzyme in Escherichia coli strain C2566/T7 | Dirofilaria immitis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Dirofilaria immitis | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Wolbachia sp. | |
| 0.55 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Wolbachia sp. |  |
| 0.55 | - |
3-phospho-D-glycerate | in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Wolbachia endosymbiont of Dirofilaria immitis | |
| 0.58 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Dirofilaria immitis | |
| 0.58 | - |
3-phospho-D-glycerate | in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Dirofilaria immitis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, calculated from amino acid sequence | Wolbachia endosymbiont of Dirofilaria immitis |
| 57000 | - |
x * 57000, calculated from amino acid sequence | Dirofilaria immitis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | Dirofilaria immitis | - |
3-phospho-D-glycerate | - |
r | |
| 2-phospho-D-glycerate | Wolbachia sp. | - |
3-phospho-D-glycerate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dirofilaria immitis | - |
- |
- |
| Dirofilaria immitis | F4ZE01 | - |
- |
| Wolbachia endosymbiont of Dirofilaria immitis | F4ZE02 | - |
- |
| Wolbachia sp. | - |
endosymbiont of filarial parasite Brugia malayi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap nickel chelating column chromatography and HiTrap Q column chromatography | Wolbachia endosymbiont of Dirofilaria immitis |
| HiTrap nickel chelating column chromatography and HiTrap Q column chromatography | Dirofilaria immitis |
| recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nicke affinity chromatography, dialysis and anion exchange chromatography | Wolbachia sp. |
| recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nickel affinity chromatography, dialysis and anion exchange chromatography | Dirofilaria immitis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimal enzyme production at 37°C | Wolbachia sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 71 | - |
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Wolbachia endosymbiont of Dirofilaria immitis |
| 71 | - |
purified recombinant His-tagged enzyme, pH 7.5, 30°C | Wolbachia sp. |
| 225 | - |
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Dirofilaria immitis |
| 225 | - |
purified recombinant His-tagged enzyme, pH 7.5, 30°C | Dirofilaria immitis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Dirofilaria immitis | 3-phospho-D-glycerate | - |
r | |
| 2-phospho-D-glycerate | - |
Wolbachia sp. | 3-phospho-D-glycerate | - |
r | |
| 2-phospho-D-glycerate | - |
Wolbachia endosymbiont of Dirofilaria immitis | 3-phospho-D-glycerate | - |
r | |
| 3-phospho-D-glycerate | - |
Dirofilaria immitis | 2-phospho-D-glycerate | - |
r | |
| 3-phospho-D-glycerate | - |
Wolbachia sp. | 2-phospho-D-glycerate | - |
r | |
| 3-phospho-D-glycerate | - |
Wolbachia endosymbiont of Dirofilaria immitis | 2-phospho-D-glycerate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, calculated from amino acid sequence | Wolbachia endosymbiont of Dirofilaria immitis |
| ? | x * 57000, calculated from amino acid sequence | Dirofilaria immitis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cofactor-independent phosphoglycerate mutase | - |
Dirofilaria immitis |
| cofactor-independent phosphoglycerate mutase | - |
Wolbachia sp. |
| independent PGM | the enzyme requires 2,3-bisphosphoglycerate as a cofactor | Wolbachia endosymbiont of Dirofilaria immitis |
| independent PGM | the enzyme requires 2,3-bisphosphoglycerate as a cofactor | Dirofilaria immitis |
| iPGM | - |
Dirofilaria immitis |
| iPGM | - |
Wolbachia sp. |
| iPGM | the enzyme requires 2,3-bisphosphoglycerate as a cofactor | Wolbachia endosymbiont of Dirofilaria immitis |
| iPGM | the enzyme requires 2,3-bisphosphoglycerate as a cofactor | Dirofilaria immitis |
| PGM | - |
Wolbachia endosymbiont of Dirofilaria immitis |
| PGM | - |
Dirofilaria immitis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Dirofilaria immitis |
| 30 | - |
assay at | Wolbachia sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 102 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Wolbachia sp. | |
| 102 | - |
3-phospho-D-glycerate | in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Wolbachia endosymbiont of Dirofilaria immitis | |
| 132 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Dirofilaria immitis | |
| 132 | - |
3-phospho-D-glycerate | in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Dirofilaria immitis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Dirofilaria immitis |
| 7.5 | - |
assay at | Wolbachia sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 2,3-bisphosphoglycerate is not required as a cofactor | Wolbachia endosymbiont of Dirofilaria immitis | |
| additional information | 2,3-bisphosphoglycerate is not required as a cofactor | Dirofilaria immitis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Dirofilaria immitis | calculated from amino acid sequence | - |
6.3 |
| Wolbachia endosymbiont of Dirofilaria immitis | calculated from amino acid sequence | - |
7.4 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 186 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Wolbachia sp. | |
| 228 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Dirofilaria immitis | |
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