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Literature summary for 5.4.2.12 extracted from

  • Saavedra, E.; Encalada, R.; Pineda, E.; Jasso-Chavez, R.; Moreno-Sanchez, R.
    Glycolysis in Entamoeba histolytica. Biochemical characterization of recombinant glycolytic enzymes and flux control analysis (2005), FEBS J., 272, 1767-1783.
    View publication on PubMed
Search for more literature for 5.4.2.12

Cloned(Commentary)

Cloned (Comment) Organism
-
 Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 2-phospho-D-glycerate pH 7.0, 37°C Entamoeba histolytica
0.106
-
 2-phospho-D-glycerate pH 6.0, 37°C Entamoeba histolytica
0.5
-
 3-phospho-D-glycerate pH 7.0, 37°C Entamoeba histolytica
0.83
-
 3-phospho-D-glycerate pH 6.0, 37°C Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 50% v/v glycerol, less than 50% loss of activity in 2 months Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Entamoeba histolytica 3-phospho-D-glycerate
-
 r
3-phospho-D-glycerate
-
 Entamoeba histolytica 2-phospho-D-glycerate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4
-
 2-phospho-D-glycerate pH 6.0, 37°C Entamoeba histolytica
14
-
 2-phospho-D-glycerate pH 7.0, 37°C Entamoeba histolytica
45
-
 3-phospho-D-glycerate pH 7.0, 37°C Entamoeba histolytica
57
-
 3-phospho-D-glycerate pH 6.0, 37°C Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6.2 formation of 2-phospho-D-glycerate Entamoeba histolytica
7
-
 formation of 3-phospho-D-glycerate Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
additional information 2,3-bisphosphoglycerate-independent phosphoglycerate mutase Entamoeba histolytica