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Literature summary for 5.4.2.12 extracted from

  • Chander, M.; Setlow, P.; Lamani, E.; Jedrzejas, M.J.
    Structural studies on a 2,3-diphosphoglycerate independent phosphoglycerate mutase from Bacillus stearothermophilus (1999), J. Struct. Biol., 126, 156-165.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of native and selenomethionyl phosphoglycerate mutase in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
methionyl and selenomethionyl phosphoglycerate mutase, vapor drop hanging diffusion, mixing equal volumes of protein solution, i.e. 50 mg/ml protein in 150 mM 3-phosphoglycerate, pH 7.4 and reservoir solution containing 2 M ammonium sulfate, 25 mM zinc acetate, 20 mM cesium chloride, 15 mM 2-mercaptoethanol, 3% polyethylene glycol 200 and 50 mM Tris-HCl, pH 7.4, crystals diffract to 2.5 A resolution Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4
-
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide pH 7.4, 1 mM Mn2+ Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required for activity Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Geobacillus stearothermophilus
-
3-phospho-D-glycerate
-
r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q9X519
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant phosphoglycerate mutase Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
500
-
selenomethionyl-phosphoglycerate mutase Geobacillus stearothermophilus
1500
-
methionyl-phosphoglycerate mutase Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Geobacillus stearothermophilus 3-phospho-D-glycerate
-
r
2-[5-amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
-
Geobacillus stearothermophilus ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
PGM
-
Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
280fold and 25fold increase in activity if pH is raised from 6.0 to 8.0 in the presence of 0.004 mM Mn2+ and 1 mM Mn2+, respectively Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
additional information 2,3-bisphosphoglycerate phosphoglycerate mutase Geobacillus stearothermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus stearothermophilus isoelectric focusing
-
6