Cloned (Comment) | Organism |
---|---|
expression of native and selenomethionyl phosphoglycerate mutase in Escherichia coli | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
methionyl and selenomethionyl phosphoglycerate mutase, vapor drop hanging diffusion, mixing equal volumes of protein solution, i.e. 50 mg/ml protein in 150 mM 3-phosphoglycerate, pH 7.4 and reservoir solution containing 2 M ammonium sulfate, 25 mM zinc acetate, 20 mM cesium chloride, 15 mM 2-mercaptoethanol, 3% polyethylene glycol 200 and 50 mM Tris-HCl, pH 7.4, crystals diffract to 2.5 A resolution | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide | pH 7.4, 1 mM Mn2+ | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required for activity | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Geobacillus stearothermophilus | - |
3-phospho-D-glycerate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9X519 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant phosphoglycerate mutase | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
500 | - |
selenomethionyl-phosphoglycerate mutase | Geobacillus stearothermophilus |
1500 | - |
methionyl-phosphoglycerate mutase | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Geobacillus stearothermophilus | 3-phospho-D-glycerate | - |
r | |
2-[5-amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide | - |
Geobacillus stearothermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
PGM | - |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
280fold and 25fold increase in activity if pH is raised from 6.0 to 8.0 in the presence of 0.004 mM Mn2+ and 1 mM Mn2+, respectively | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 2,3-bisphosphoglycerate phosphoglycerate mutase | Geobacillus stearothermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus stearothermophilus | isoelectric focusing | - |
6 |