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Literature summary for 5.4.2.12 extracted from
- Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus (2000), EMBO J., 19, 1419-1431.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Geobacillus stearothermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals of iPGM complexed with 3-phosphoglycerate and Mn2+ at 1.9 A resolution | Geobacillus stearothermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D12N | no activity | Geobacillus stearothermophilus |
| H123N | 3% of wild-type activity | Geobacillus stearothermophilus |
| H125N | 67% of wild-type activity | Geobacillus stearothermophilus |
| H128N | 20% of wild-type activity | Geobacillus stearothermophilus |
| H407N | no activity | Geobacillus stearothermophilus |
| H42N | 91% of wild-type activity | Geobacillus stearothermophilus |
| H42N/H128N | 8% of wild-type activity | Geobacillus stearothermophilus |
| H445N | 18% of wild-type activity | Geobacillus stearothermophilus |
| H462N | 0.3% of wild-type activity | Geobacillus stearothermophilus |
| H66N | 2% of wild-type activity | Geobacillus stearothermophilus |
| R261L | no activity | Geobacillus stearothermophilus |
| S62A | no activity | Geobacillus stearothermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | absolutely required for activity, maximal activation at 1 mM | Geobacillus stearothermophilus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | Geobacillus stearothermophilus | - |
3-phospho-D-glycerate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | Q9X519 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant iPGM | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Geobacillus stearothermophilus | 3-phospho-D-glycerate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| iPGM | - |
Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 2% and 22% of maximal activity at pH 6.0 and pH 7.0, respectively, at 1 mM Mn2+ | Geobacillus stearothermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 2,3-bisphosphoglycerare independend PGM | Geobacillus stearothermophilus |
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