Literature summary for 5.4.2.11 extracted from

de Atauri, P.; Repiso, A.; Oliva, B.; Vives-Corrons, J.L.; Climent, F.; Carreras, J.
Characterization of the first described mutation of human red blood cell phosphoglycerate mutase (2005), Biochim. Biophys. Acta, 1740, 403-410.

Search for more literature for 5.4.2.11

Application

Application	Comment	Organism
medicine	mutation M230I is identified in a patient with hereditary sperocytosis and 50% deficiency of enzyme activity in red blood cells. Enzyme shows abnormal behaviour on ion-exchange chromatography and is more thermo-labile than wild-type	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
M230I	mutation identified in a patient with hereditary sperocytosis and 50% deficiency of enzyme activity in red blood cells. Enzyme shows abnormal behaviour on ion-exchange chromatography and is more thermo-labile than wild-type	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.4	-	3-phospho-D-glycerate	pH 7.4, 30°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	patient with hereditary sperocytosis and 50% deficiency of enzyme activity in red blood cells	-

Reaction

Reaction	Comment	Organism	Reaction ID
2-phospho-D-glycerate = 3-phospho-D-glycerate	ping-pong or phosphoenzyme mechanism	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	hemoglobin-free hemolysate	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Homo sapiens	3-phospho-D-glycerate	-	r
3-phospho-D-glycerate	-	Homo sapiens	2-phospho-D-glycerate	-	r
additional information	enzyme possesses some 2,3-bisphosphoglycerate phosphatase activity	Homo sapiens	?	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	-	wild-type, 1 h, 10% loss of activity, mutant M230I, 20 min, 50% loss of activity, 1 h, 90% loss of activity. In presence of 2,3-bisphosphoglycerate, both forms remain stable	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)