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Literature summary for 5.4.2.10 extracted from
- Reaction mechanism of phosphoglucosamine mutase from Escherichia coli (1999), Eur. J. Biochem., 262, 202-210.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-glucosamine 1,6-diphosphate | retains the enzyme in an active and phosphorylated form | Escherichia coli |  |
| D-glucose 1,6-diphosphate | retains the enzyme in an active and phosphorylated form | Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S100A | very low activity | Escherichia coli |
| S100T | contributes to the specificity towards sugar- or aminosugar-phosphates, much more efficient in catalysis of the phosphoglucose mutase reaction | Escherichia coli |
| S102A | site of phosphorylation, no activity | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
D-glucosamine 6-phosphate | - |
Escherichia coli | |
| 0.08 | - |
D-glucosamine 1-phosphate | - |
Escherichia coli | |
| 0.08 | - |
D-glucosamine 1,6-diphosphate | - |
Escherichia coli | |
| 0.08 | - |
D-glucose 1-phosphate | single mutation S100T | Escherichia coli | |
| 0.5 | - |
D-Glucose 1,6-bisphosphate | - |
Escherichia coli | |
| 0.65 | - |
D-glucose 1-phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47412 | - |
3 * 47412, gel filtration, mass spectrometry | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucoamine 6-phosphate | Escherichia coli | ping-pong bi-bi reaction mechanism | D-glucosamine 1-phosphate | - |
r | |
| D-glucoamine 6-phosphate | Escherichia coli | two basically different reaction sequences, 1. phosphate group transfer yielding the intermediate D-GlcN-1,6-diphosphate, 2. conversion to GlcN-1-phosphate | D-glucosamine 1-phosphate | - |
r | |
| D-glucoamine 6-phosphate | Escherichia coli | involved in formation of cell-wall precursor UDP-N-acetylglucosamine | D-glucosamine 1-phosphate | - |
r | |
| D-glucosamine 1-phosphate | Escherichia coli | - |
D-glucosamine 6-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the only phosphogroup at Ser102 | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| near homogeneity | Escherichia coli |
| near homogeneity, recombinant enzyme | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.007 | - |
substrate: D-glucose 6-phosphate | Escherichia coli |
| 3 | - |
6x His-tagged recombinant enzyme | Escherichia coli |
| 10 | - |
substrate: D-glucosamine 6-phosphate | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucoamine 6-phosphate | ping-pong bi-bi reaction mechanism | Escherichia coli | D-glucosamine 1-phosphate | - |
r | |
| D-glucoamine 6-phosphate | two basically different reaction sequences, 1. phosphate group transfer yielding the intermediate D-GlcN-1,6-diphosphate, 2. conversion to GlcN-1-phosphate | Escherichia coli | D-glucosamine 1-phosphate | - |
r | |
| D-glucoamine 6-phosphate | involved in formation of cell-wall precursor UDP-N-acetylglucosamine | Escherichia coli | D-glucosamine 1-phosphate | - |
r | |
| D-glucosamine 1,6-diphosphate | - |
Escherichia coli | ? | - |
? | |
| D-glucosamine 1-phosphate | - |
Escherichia coli | D-glucosamine 6-phosphate | - |
r | |
| D-glucose 1,6-bisphosphate | - |
Escherichia coli | ? | - |
? | |
| D-glucose 1-phosphate | in the presence of Glc-1,6-diphosphate, 1400fold lower activity than for D-glucosamine 1-phosphate or D-glucosamine 1,6-diphosphate, ping-pong reaction mechanism | Escherichia coli | D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 47412, gel filtration, mass spectrometry | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0055 | - |
D-glucose 1-phosphate | - |
Escherichia coli | |
| 0.0138 | - |
D-glucose 1-phosphate | single-mutation S100T | Escherichia coli | |
| 2.42 | - |
D-glucosamine 6-phosphate | 6 x His-tagged recombinant enzyme | Escherichia coli | |
| 7.9 | - |
D-glucosamine 6-phosphate | - |
Escherichia coli | |
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